Introduction: In most cases, colon cancer originates from intestinal polyps, which usually do not cause any specific clinical symptoms in the body, but turn into cancer over time. Colon polyps are lumps of flesh that originate from the inner lining of the colon. A person with an intestinal polyp may not have any symptoms or discomfort. But these polyps may turn into cancer if not treated. Colon cancer almost always originates from polyps or premalignant lesions of the intestine. Therefore, polyps should be removed from the colon to prevent cancer.Treatments for colon cancer can include a combination of surgery, radiation, chemotherapy, and targeted therapy.
colorectal Cancer(CRC) is on of the most common malignant diseases in the world,and it's incidences increased with age.The signs and symptoms of this disease can include things such as blood in the stool (hematochezia), changes in bowel movements and bowel movements, weight loss, and constant fatigue.
Methods: First GSE(185770) were downloaded from NCBI GENE Expression omnibus(GEO). GEPIA2 and ENCORI analyzed the data sets to find out differentially expressed genes in patients.Pathway enrichment was performment KEGG.We usedfor the probability cause of increased expression SNPs located on the 3'UTR region.from the STRING site for interaction pro-pro and intraction miRNA-MRNA from mirwalk database and intraction LncRNA-miRNA from the LncRRisearch and intraction mRNA-LncRNA from the Lncbase3 search it.
Results: CXCL8 had a significant high expression in the tumor samples.(logFc=2.79 ,adj=1.537e_02).Also in databases GEPIA2 and ENCORI expression increase in cancer cells was significant.CXCL8 is in Malaria pathway. This has-MIR 374a-3p suppresses the CXCL8 gene.and we found by blacing diffrent genes HELLPAR Lnc RNA.
HELLPAR Lnc RNA intraction was observed in CeRNA.rs769138009 in gene 3'UTR cause target loss.
Conclusion: As a result of this study, concluded that HELLPAR LncRNA act as a tumor suppressor in colorectal cancer(CRC) by inhibiting the function of the has-mir-374a-3p mirRNA so it blocks miRNA sponging and eventually represses the CXCL8 gene which is an important gene for metabolism and biosynthesis.therefore,this result may be considered as potenial therapeutic purpose for CRC patients.
Keywords: Biomarker, Microarray analysis, RNA interacton, Systems Biology