based on gene expression profiling and systems miR-28-5p regulates the expression level of GLUD1 gene in multiple sclerosis patients: a study biology analyses
based on gene expression profiling and systems miR-28-5p regulates the expression level of GLUD1 gene in multiple sclerosis patients: a study biology analyses
Faezeh Rezaei,1Mohammad Rezaei,2Mrs mansourehazade,3,*
1. Zist Fanavari Novin Biotechnology Institute, Isfahan, Iran 2. ZistFanavari Novin Biotechnology Institute, Isfahan, Iran 3. Zist Fanavari Novin Biotechnology Institute, Isfahan, Iran
Introduction: Introduction: MS is a disease related to the nervous system that can affect the brain and spinal cord. This condition causes a wide range of potential symptoms, including vision problems, problems with hand or foot movement, and emotional problems. In this disease, the body's immune system mistakenly It attacks its natural tissues.
Methods: Methods: expression (GEO) and then analyzed by GEO2R to find differentially expressed genes by the target gse21924. The gene was selected from the David site in the kegg-pathway section. In the next step, we use the mir-walk site of the desired gene and select Mir. On the DIANA Tool site, we select the miRNA-IncRNA section and execute the search for the desired miR.The next step is to examin the relationship between the lnc genes, use the lncRRISEARCH site. Finally, from the kegg site, we find the signaling pathway in which the desired gene is involved, and from the string site, it enters the desired gene that we have chosen, and then gives us the proteins that are related to it
Results: Results: The GLUD1 (logFC: 1.988, adj.P.Val: 0.005387) could regulate the multiple sclerosis progression as a high expressed mRNA in the patients. MiR-28a-5p (score: 1, energy: 0.95, 3utr) and lncRNA A1BG-AS1 (interaction energy: -12.06 kcal/mol) regulates the expression level ofGLUD1 in the regulation of Nitrogen metabolism signaling pathways. Based on the protein-protein interaction analysis, GPT2 and GLS2 proteins have significant interaction with GLUD1 Mentioned proteins could regulate the MS status by modulating the Nitrogen metabolism signaling pathway.
Conclusion: Conclusion: miR-28-5p and lncRNA A1BG-AS1 regulate the expression level GLUD1 in regulating the Nitrogen metabolism signaling pathways in Multiple Sclerosis patients. High expression of GLUD1 protein increases Multiple Sclerosis development risk, and miR-28-5p and lncRNA A1BG-AS1can control this dysregulation.