Co-treatment of Alpha-Lipoic Acid and Injectable platelet rich fibrin (i-PRF) bioscaffold can reduction of oxidative stress in mouse ovarian tissue following autotransplantation
Co-treatment of Alpha-Lipoic Acid and Injectable platelet rich fibrin (i-PRF) bioscaffold can reduction of oxidative stress in mouse ovarian tissue following autotransplantation
sahar hatami,1,*Seyed Mohammadali Shariatzadeh,2Malek Soleimani Mehranjani,3
1. Phd student.Department of Biology, Faculty of Science, Arak University, Arak, Iran. 2. Department of Biology, Faculty of Science, Arak University, Arak, Iran. 3. Department of Biology, Faculty of Science, Arak University, Arak, Iran.
Introduction: However, Ovarian tissue transplantation is best option for cancerous young woman. But, ischemia-reperfusion (I/R) injury remains the most important limitation of this method and Occurrence of oxidative stress then reduces the survival of autografted tissue. . Injectable platelet rich fibrin (i-PRF) is a liquid formulation of platelet rich fibrin (PRF) without the use of anti-coagulants. It contains a fibrin matrix resulted from fibrinogen molecules activation in plasma, leukocytes, circulating stem cells, platelets and growth factors. On the other hand Alpha-lipoic acid (ALA) is a powerful antioxidant that can inhibit oxidative stress and cell damage. In this study, we investigated the effect of co-treatment i-PRF bioscaffold and ALA on the Serum concentrations of malondialdehyde (MDA) and total antioxidant capacity (TAC) after mouse ovarian tissue transplantation.
Methods: Experimental samples consisted of three groups (n=6): control, autograft + saline (whole ovarian tissue transplanted in the gluteus superficialis muscle, saline directly injected into it), autograft + i-PRF+ ALA (first, the mice received 100 mg/kg" intraperitoneal injections of ALA, 30 minutes before transplantation than whole ovarian tissue transplanted in the gluteus superficialis muscle, i-PRF was directly injected into it ). 7 days after ovary transplantation, the serum level of MDA and TAC were assayed. We Data analyzed dy one-way ANOVA and Tuckey’s test and the means were considered significantly different at p-value < 0.05.
Results: In the the control group, the serum level of MDA significantly decreased in compare to other groups, while it showed a significant increase in the autograft group compared to the autograft + i-PRF+ ALA group (p < 0.05). Moreover, Serum concentrations of TAC decreased significantly in the autograft group compared to the control counterpart, whereas it increased significantly in the autograft + i-PRF+ ALA group compared to the autograft group (p < 0.05).
Conclusion: This results for the first time showed that concomitant use i-PRF bioscaffold and ALA can prevent IR damages in mice transplanted ovaries through reduction oxidative stress and improves thier function.