• Evaluation of genome extraction using magnetic nanoparticles
  • Seyed Mahdi Hosseinian,1 Seyed Reza Mohebbi,2,* Seyed Masoud Hosseini,3 Paria Barooni,4 Shabnam Kazemian,5 Hamid Asadzadeh-Aghdaei,6
    1. Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
    2. Research Center for Gastroenterology and Liver Diseases, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
    3. Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
    4. Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
    5. Research Center for Gastroenterology and Liver Diseases, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
    6. Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran


  • Introduction: Genome isolation is a prerequisite for many molecular biology and genetic engineering techniques. Since the discovery of DNA and RNA, various methods have been developed and used to extract genomic content, and to date, efforts have been made to reduce contamination and optimize extraction time, quality and concentration.
  • Methods: In September 2022, our research was conducted on the ScienceDirect, PubMed, SID, and Scopus databases. Articles with irrelevant titles or abstracts were excluded. Finally, the most related articles were selected.
  • Results: Recent studies have shown the high efficiency of coated magnetic nanoparticles, especially silica-coated, compared to bare or uncoated nanoparticles.
  • Conclusion: Genome extraction using magnetic nanoparticles is an easy, fast, and efficient method that can provide a good quality nucleic acid product. In addition, the efficiency of magnetic nanoparticles can be greatly increased by improving the performance of magnetic separation, improving stability, and adding special functional groups.
  • Keywords: Magnetic nanoparticles, Genome extraction, Magnetic Separation, PCR, Fe3O4@SiO2