• The therapeutic effects of hAMSCs secretome on tumor invasion through down regulation of SgK269 in HT-29 cells colon cancer cells
  • Fatemeh Ansari Dogaheh,1,* Fatemeh Safari,2
    1. Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran
    2. Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran


  • Introduction: Colon cancer is one of the leading tumors in the world, killing many people each year. Currently, the most common types of cancer treatment are surgery, chemotherapy, immunotherapy and radiotherapy. Due to drug resistance in cancer therapy and toxicities effects of anticancer agents on normal cells, researchers are always interested to find novel, safe and more effective strategies to design anticancer drugs. Stem cells with unique characteristics such as self-renewal, directional migration, differentiation, and modulatory effects on other cells, are considered as a new platform in cancer therapy and repair of damaged tissues. Atypical kinase Sugen Kinase 269 (SgK269) was reported to regulate invasion and cell motility. The aim of present study was to evaluate the therapeutic effects of human amniotic mesenchymal stromal cells (hAMSCs) on colon cancer cells (HT-29) invasion through analyzing SgK269 expression.
  • Methods: To do so, we employed a co-culture system using 6 well plates transwell with a diameter of 0.4 μm pore sized. After 72h hAMSCs-treated HT-29 cells, the expression of SgK269 was analyzed by using western blot method.
  • Results: Our results support of the idea that MSCs may be a novel and more effective therapeutic approach to inhibit colon cancer cells invasion by targeting specific gene expression.
  • Conclusion: It seems that hAMSCs may be a nobel and potential platform in colon cancer therapy. However, more experiments are required to determine the details of related molecular pathways.
  • Keywords: HT-29 cells colon cancer cells, hAMSCs, SgK269, Invasion