• Evaluation of the effect of Staphylococcus aureus cytoplasmic extract on U87 glioblastoma cell line and evaluation of the expression of apoptotic bax and bcl-2 genes
  • Maryam Rasouli,1,* Bahareh Hajikhani,2 Hossein Fahimi,3
    1. Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
    2. Department of Medical Microbiology, Shahid Beheshti University of Sciences, Tehran, Iran.
    3. Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran


  • Introduction: Glioblastoma is one of the most common malignant diseases that develop in the spinal cord or brain, and its prevalence rate in Iran is faster than the global statistics of a decade and is one of the most serious causes of death in patients. So far, many treatment methods have been used for treatment, but these methods were transient, there was still a possibility of disease recurrence and they did not have a good effect on the patient's survival.In recent years, drugs such as bacterial toxins, different fractions, and cytoplasmic extracts have received attention in the treatment of various types of cancers. Therefore, the purpose of this study is to evaluate the effect of Staphylococcus aureus cytoplasmic extract and its function on U87 glioblastoma cell line and its effect on apoptosis induction.
  • Methods: U87 cell line cells were produced from Iran's teak reserves center and the cells were grown in suitable conditions. Staphylococcus aureus was also cultured in LB Broth at 37 degrees for 24 hours; Then, plant cytoplasmic extract was prepared by sonication and its contents were evaluated by SDS-PAGE. Lowry's method was used to determine and finally, the inhibitory effect of Staphylococcus aureus on cell proliferation and apoptosis was investigated through MTT test. The tumor cell was treated with an ideal dose of Staphylococcus aureus extract, and the expression rate of bcl-2 and bax genes was measured using Real-Time-PCR techniques.
  • Results: Cytoplasmic extract of bacteria prevented the continuation of the proliferation process in U87 cell line and induced apoptosis by increasing the expression of apoptotic gene bax and decreasing the expression of bcl-2 gene. In fact, based on the analysis performed between the case and control groups by means of a statistical test, it is observed that the expression of bcl-2 in the treatment cDNA cell based on the CT ranking is lower than the control group with a significant difference in variance, and also the expression of the bax gene in a sample of has been treated Attention to the CT rate is higher than the control group (P Value < 0.05).
  • Conclusion: These results proved that the cytoplasmic extract of Staphylococcus aureus has an inhibitory effect on proliferation in the U87 cell line, and by implementing more projects in the direction of identifying bacterial substances with anticancer properties, Staphylococcus aureus extract can be used. It has been named as a favorable candidate for inducing apoptosis in malignant tumors.
  • Keywords: Glioblastoma, Staphylococcus aureus, apoptosis, cell proliferation, U87 cell line