Detection of aminoglycoside resistance genes in Enterococcus faecalis strains isolated from a clinical sample in Shahrekord
Detection of aminoglycoside resistance genes in Enterococcus faecalis strains isolated from a clinical sample in Shahrekord
Parisa behshood,1,*Fateme Talebi varnosfaderani,2Mozhgan Kameli khouzani,3Elahe Tajbakhsh,4
1. PhD, Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran 2. PhD, Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran 3. PhD, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran 4. PhD, Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
Introduction: Background and Objectives: Today Enterococci resistance to some drugs has gained widespread that it would cause complications in the treatment of infections caused by these bacteria. The purpose of this study was the evaluation of resistance to aminoglycoside antibiotics in Enterococcus faecalis by multiplex PCR.
Methods: 130 suspects Enterococcus isolates were collected from laboratory Shahrekord. Bacteria were identified by routine microbiological tests. Susceptibility testing towards aminoglycosides antibiotic using disk diffusion method. Finally, Multiplex PCR assays were performed to identify aminoglycosides antibiotic resistance genes.
Results: Out of the 130 isolates, 75 isolates were Enterococcus faecalis. The highest resistance towards Tetracycline and Streptomycin and the highest sensitivity to Vancomycin. PCR results showed the prevalence of aac (6')-Ie- aph (2")-Ia,ant(4')-Ia and aph (3') – IIIa, genes to be in order of %60, %26/6, and %56 , respectively. Ten isolates possessed all three genes.
Conclusion: According to the results of high-level antibiotic resistance, periodic tracking of antibiotic resistance genes in clinical samples is recommended.