Antibacterial effect of alcoholic and aqueous extract of Propolis on Staphylococcus aureus and Pseudomonas aeruginosa
Antibacterial effect of alcoholic and aqueous extract of Propolis on Staphylococcus aureus and Pseudomonas aeruginosa
Fatemeh Talebi Varnosfaderani,1,*Parisa Behshood1,2Mozhgan Kameli khouzani,3Zahra Bamzadeh3,4Hassan Momtaz,5
1. Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran 2. Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran. 3. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. 4. Department of Microbiology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran 5. Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
Introduction: Pseudomonas aeruginosa and Staphylococcus aureus can usually cause disease in humans. Propolis extract is known as an antimicrobial compound that affects bacteria. This research aims to determine the antimicrobial activity of propolis extract on bacteria strains in vitro and the pattern of antibiotic resistance.
Methods: In this research, the antibacterial effects of propolis alcoholic extract on Pseudomonas aeruginosa and Staphylococcus aureus bacteria (Kirby-Bauer method) and dilution in the tube, determining the Minimum Inhibitory Concentration (MIC) and the Minimum Bacteria Concentration (MBC) was evaluated. SPSS was used for statistical analysis.
Results: Based on the results, antimicrobial activity, non-growth halo diameter of Pseudomonas aeruginosa and Staphylococcus microorganisms were determined, and Staphylococcus aureus showed the highest sensitivity and Pseudomonas aeruginosa showed the highest resistance to propolis alcoholic extract.
Conclusion: The results of the antibiotic resistance pattern showed that Pseudomonas aeruginosa is only sensitive to gentamicin, while Staphylococcus aureus is resistant to most antibiotics. Conclusion: The alcoholic extract of propolis hadn't only an inhibitory effect but also antibacterial properties on Pseudomonas aeruginosa and Staphylococcus aureus bacteria.