Comparison Achillea millefolium extract antimicrobial action and antibiotic resistance isolates Staphylococcus aureus and Enterococcus feacalis from clinical and standard samples in Isfahan Hospital
Comparison Achillea millefolium extract antimicrobial action and antibiotic resistance isolates Staphylococcus aureus and Enterococcus feacalis from clinical and standard samples in Isfahan Hospital
Fatemeh Talebi Varnosfaderani,1,*Parisa Behshood,2Mozhgan Kameli khouzani,3
1. Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran 2. Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran. 3. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Introduction: S.aureus and E.fecalis are important factors that cause disease in people, while antibiotic resistance increase rapidly in the world in recent years. Then researchers trend toward herbal cures. The aim of this study, check the antimicrobial activity of A.millefolium extract and antibiotic resistance for S.aureus and E.fecalis.
Methods: Achillea millefolium was provided and dried. Then extract methanol, ethanol, and Easton extract from the plant. Standard samples included extract S.aureus and E.feacalis collected from IROST. A total of 6 clinical specimens (3 S.aureus and 3 E.feacalis) were taken from Isfahan hospital and identified all isolates. Antibiotic resistance was assessed using disk diffusion method for Vancomycin, Cefazolin, Gentamicin, Tetracyclin, Ciprofloxacin, Oxacillin, and Trimethoprim-Sulfametaxole. Kirby-Bauer antibiogram conventional method was performed for the determination of antibiotic resistance bacteria. Antibacterial activity of extracts was evaluated by disk diffusion method, Concentration of herbals that inject was 0/5, 0/250, 0/125,0/0625 mg/ml.
Results: Most S.aureus are resistant to Cephazolin, Tetracycline, Oxacillin, and Vancomycin, and E.fecalis show resistance to Cephazolin and Vancomycin. Herbal extracts affect bacteria but not as much as antibiotics.
Conclusion: In this study, samples showed vancomycin and cefazolin resistance, which was an alarm of the most important reason for antibiotic resistance used a lot of them by people, especially B-Lactam antibiotics cause resistance to several drugs. To prevent and decrease antibiotic resistance must use antibiotics under supervision with suitable doses. Various concentrations of methanol, ethanol and acetone extracts have antibacterial resistance but it was so little. The results showed that A.millefolium hadn’t enough effect on gram-positive bacteria, maybe it affects gram-negative bacteria. Suggested examination extract of A.millefolium on gram-negative bacteria.