Investigating antimicrobial effect of the concentrated extract powder of Scrophularia striata plant on Streptococcus mutans and Streptococcus sobrinus strains
Investigating antimicrobial effect of the concentrated extract powder of Scrophularia striata plant on Streptococcus mutans and Streptococcus sobrinus strains
Saeed feyzbabaei cheshmeh sefid,1,*
1. student vet Laboratory Science, Faculty of Para Veterinary Medicine, Ilam University - Iran - Iran
Introduction: acteria in the oral cavity, including Streptococcus species, can be an important cause of tooth decay and plaque in the presence of predisposing factors. This problem causes most people in the society to spend a lot of money on dentistry every year. Therefore, in order to prevent and treat properly, using a low-cost and safe method based on medicinal plants can increase the general health of the mouth and teeth. On the other hand, the consumption of natural compounds is also very important in preventing the emergence of antibiotic resistance of microorganisms that cause oral and dental infections. Therefore, the purpose of this study is to investigate the antibacterial effect of this valuable plant and to find a mouthwash formula based on medicinal plants and traditional medicine for oral and dental health.
Methods: In this research, first, extracting from the plant (S. striata) was done by maceration. Then, by using rotary devices and spray dryers, the aqueous extract was concentrated and powdered, and serial concentrations were also prepared from the concentrated extract powder. Finally, the minimum inhibitory and lethal concentration (MIC and MBC) concentrations of 20, 30, 40, 50, 60, 70, 80 mg/100 ml prepared from the mentioned plant, on the standard strains of Streptococcus mutans ATCC 35668 and Streptococcus subrinus ATCC 27607 was investigated.
Results: After exposing the bacteria to different concentrations of the extract, the results of their culture on tryptose soy agar medium show that the minimum inhibitory concentration for Streptococcus mutans is 40 mg and the minimum lethal concentration is 60 grams, and for Streptococcus subrinus The minimum inhibitory concentration was reported to be 30 gr/ml and the minimum lethal concentration was 50 mg/ml for this strain.
Conclusion: According to the results obtained in this research, the extract of Scrophularia striata plant can be used to make mouthwashes and effective drugs in controlling oral infections and tooth decay.