Flaxseed biocoumpunds modified the TNF-α/IL6/SIRT1/NRF1/NPPA/FGF7 network in Myocardial infarction
Flaxseed biocoumpunds modified the TNF-α/IL6/SIRT1/NRF1/NPPA/FGF7 network in Myocardial infarction
Navid Abedpoor,1Fatemeh Hajibabaie,2Kamran Safavi,3,*
1. Department of Physiology, Medicinal Plants Research Center, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran. 2. Department of Physiology, Medicinal Plants Research Center, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran. 3. Department of Plant Biotechnology, Medicinal Plants Research Centre, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
Introduction: Cytokine storms and cytokine release syndrome (CRS) lead to cardiovascular disease. Phytochemicals and bioactive compounds of the herbals are vital sources of active agents and have potential approaches to preventing or therapy cardiovascular disease. Flaxseed contains phytochemicals, including secoisolariciresinol diglucoside, lignans, and linolenic acid (SAL), with anti-inflammatory and antioxidant activity.
Methods: Here, candidate hub genes, TNF-α, IL6, SIRT1, NRF1, NPPA, and FGF7, were selected based on in-silico analysis and artificial intelligence. Myocardial infarction (MI) was induced using H9c2 cardiac cells in hyperlipidemic and hyperglycemic conditions. RT-qPCR was conducted to evaluate the expression of genes.
Results: This study indicated that SAL compounds bound to the Il-6, SIRT1, and TNF-α as a druggable candidate protein based on the chemoinformatics analysis. This study indicated that the deregulation of TNF-α, IL6, SIRT1, NRF1, NPPA, and FGF7 networks in MI models and SAL compounds ameliorated the expression level of these genes. Furthermore, SAL compounds improved the function and myogenesis of H9c2 cardiac cells in hyperlipidemic and hyperglycemic conditions.
Conclusion: Moreover, our data indicated that phytochemicals obtained from flaxseed might have potential complementary treatment or preventive strategies for MI.