• Association between Mir32 rs7041716 Polymorphism and Breast Cancer in Iranian Women
  • Seyedeh Zahra Amirabadi,1 Morteza Abkar,2 Shahrzad Ahangarzadeh,3 Parisa Shoaei,4 Saghar Yousefnia,5,*
    1. Department of Genetics, Faculty of Biological Sciences and Technology, Shahid Ashrafi Esfahani University, Isfahan, Iran.
    2. Department of Genetics, Faculty of Biological Sciences and Technology, Shahid Ashrafi Esfahani University, Isfahan, Iran.
    3. Infectious and tropical diseases research center Isfahan University of Medical Sciences Assistant Professor Medical Biotechnology
    4. Infectious and tropical diseases research center of Isfahan University of Medical Sciences
    5. Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran. Department of Genetics, Faculty of Biological Sciences and Technology, Shahid Ashrafi Esfahani University, Isfahan, Iran.


  • Introduction: About 5–10% of breast cancers (BC) are considered hereditary, from which the known BC genes account for 3–4%. Since mutations that are known to increase breast cancer risk within families are quite rare, identification of polymorphisms related to the disease is very important. Among the different biomarkers, polymorphism in Mir genes has attracted a lot of attention. In present study, association between Mir32 rs7041716 polymorphism and BC was examined in Iranian women population.
  • Methods: At first, sampling was done from 100 women with BC and 105 normal women. Then DNA was isolated from the samples. The quality and quantity of extracted DNA was determined using electrophoresis on agarose gel and nanodrop device. Finally, by using specific primers and tetra-primer ARMS PCR method, the rs7041716 (A > C) was investigated.
  • Results: The results of electrophoresis on agarose gel and nanodrop device showed that the isolated DNA has good quality and quantity. Statistical analysis revealed that the frequency of C allele and CC genotype in women with BC is higher than normal women. In addition, odd ratio analysis revealed that the C allele in the heterozygote genotype may increase the risk of BC as 2.9-fold.
  • Conclusion: According to the obtained results, the Mir32 Rs7041716 polymorphism can be used as a genetic marker in the diagnosis of BC.
  • Keywords: Breast cancer, MIR32, Tetra-arms PCR, rs7041716.