the evaluation of LYL1 gene in Acute lymphoblastic leukemia in children
the evaluation of LYL1 gene in Acute lymphoblastic leukemia in children
kamal shahamiri,1,*
1. Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
Introduction: In recent years, an increasing trend in the incidence of acute lymphoblastic leukemia (ALL) has been reported. However, the molecular mechanisms involved are not fully understood. The aim of this study was to evaluate the expression of LYL1 gene in patients with Acute lymphoblastic leukemia for the identification of the gene expression changes so that the role of the gene in diagnosis and treatment of the disease could be determined
Methods: This case-control study was performed on 40 ALL patients and 40 healthy controls in the years 2020-2021. For this purpose, total RNA was extracted from blood samples and after cDNA synthesis, LYL1 expression was measured using Real-Time PCR. Statistical analysis of the results was performed using SPSS software and appropriate tests
Results: The results of the gene expression study showed that in patients with ALL, LYL1 expression compared to controls had significant increases. These expression changes were not significantly different in age, sex, MRD, and T-ALL and B-ALL categories
Conclusion: In conclusion, downregulation or upregulation of LYL1 may be of importance in the biology of ALL .Other studies are also required to elucidate the exact function of this gene in cancer