Changing oxidative stress parameters in the testis of depressed rats under hesperidin treatment
Changing oxidative stress parameters in the testis of depressed rats under hesperidin treatment
Maryam Azimi,1Homeira Hatami,2,*Sama Yavari,3Mahsa Otarkaniy,4
1. Changing oxidative stress parameters in the testis of depressed rats under hesperidin treatment 2. Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
Introduction: Depression is a current and disabling disease. The prolonged psychological stress is a causative factor for depression, which leads to increase in MDA levels, oxidative stress, and depressive symptoms. Depression causes hormonal imbalance which can affect the testicles. Extra ROS production and exhaustion of anti-oxidative defenses trigger pro-inflammatory. The levels of different oxidative stress markers (MDA, SOD) are found to be altered in depressive disorders MDA status is used as a biomarker for oxidative stress. The study aims to investigate the effects of hesperidin on MDA and SOD factors of oxidative stress in the testis tissue of depressed rats.
Methods: In this experimental study, we used 24 adult male rats. The animals were divided into four groups. The control groups that received saline for 14 days, the depressed group that received reserpine (0.5mg/kg) for 14 days, the hesperidin group that received hesperidin (20mg/kg) for 14 days, and the depressed group was treated with hesperidin, they first received reserpine for 14 days for induction of depression and then received the hesperidin (20mg/kg) for 14 days. In this study, a forced swimming behavior test was performed to confirm the induction of depression. 24 hours after the last injection, animals were sacrificed and the level of MDA and SOD were measured in the testis. The basis of the tissue MDA measurement method is based on reaction with thiobarbituric acid (TBA), and extraction with butanol. Data were analyzed by using One-Way ANOVA.
Results: The results showed that in the forced swimming test, as an index of depression induction, the mobility time increased significantly between the depressed group and the hesperidin treatment group (p<0.05). The level of MDA has changed significantly between groups treated with hesperidin and the control group
The result shows the level of SOD between groups was not changed significantly.
Conclusion: It seems that hesperidin as an antioxidant can improve the MDA factor in oxidative stress in depressive rats.