مقالات پذیرفته شده در هفتمین کنگره بین المللی زیست پزشکی
Investigating the effect of radiotherapy and sonodynamic therapy in the presence of nanoparticle on breast cancer cells
Investigating the effect of radiotherapy and sonodynamic therapy in the presence of nanoparticle on breast cancer cells
Zeinab Hormozi Moghaddam,1,*Ali Neshastehriz,2seyed Mohammad Amini,3Zahra abedi kichi,4
2. 1. Radiation sciences department, Iran University of Medical Sciences (IUMS), Tehran, Iran. 2. Radiation Biology Research center, Iran University of Medical Sciences (IUMS), Tehran, Iran. 3. 1. Radiation sciences department, Iran University of Medical Sciences (IUMS), Tehran, Iran. 2. Radiation Biology Research center, Iran University of Medical Sciences (IUMS), Tehran, Iran. 4. 3. Department of Genetics, Faculty of biological Sciences, Tarbiat Modares University, Tehran, Iran. 4. Institute for Cardiovascular prevention (IPEK), Ludwig-Maximilians University Munich, Germany.
Introduction: Introduction: Sonodynamic therapy and nanoparticles with green compounds, along with radiotherapy, could reduce complications and improve the treatment of cancer. This study aimed to investigate the efficacy of low-dose radiotherapy and sonodynamic therapy in the presence of apigenin-coated gold nanoparticles on the breast cancer cell.
Methods: Methods and materials: The synthesized apigenin-coated gold nanoparticles were confirmed by UV-visible, HR-TEM, DLS, zeta-potential, and FTIR. Ultrasound parameters were estimated at the acoustic cavitation threshold by mechanical index modeling. The toxicity of the nanoparticles and methylene blue were evaluated by MTT. The concentration of apigenin-coated gold nanoparticles was measured, and they were then irradiated by ultrasound and 2 Gy x-ray radiation.
Results: Results: Ultrasound with a mechanical index of 0.40 was estimated at a distance of 2 cm from the 1-MHz transducer with 2 W/cm2 in the continuous mode. The MTT assay indicated that sonodymic therapy combined with radiation therapy at the concentration of 8 µg/ml nanoparticles significantly affected cell death (0.26± 0.02).
Conclusion: Conclusion: Using sonodynamic therapy as a non-invasive and non-ionizing treatment with novel sensitizers based on optimum physical parameters can increase cell death in low-dose radiation therapy.