مقالات پذیرفته شده در هفتمین کنگره بین المللی زیست پزشکی
Evaluation of antibiotic susceptibility, biofilm formation ability, prevalence of extended-spectrum beta-lactamase (ESBL), multi-drug resistant (MDR) in clinically isolates Escherichia coli and Klebsiella pneumoniae
Evaluation of antibiotic susceptibility, biofilm formation ability, prevalence of extended-spectrum beta-lactamase (ESBL), multi-drug resistant (MDR) in clinically isolates Escherichia coli and Klebsiella pneumoniae
Mina Shirmohammadpour,1Hanieh Safarzadeh,2Bahman Mirzaei,3,*
1. Department of Microbiology and Virology, Zanjan University of Medical Sciences, Zanjan, Iran 2. Department of Microbiology and Virology, Zanjan University of Medical Sciences, Zanjan, Iran 3. Department of Microbiology and Virology, Zanjan University of Medical Sciences, Zanjan, Iran
Introduction: Escherichia coli and Klebsiella pneumoniae are opportunistic bacteria with high prevalence and antimicrobial resistance (AMR). The sensitivity pattern and biofilm formation ability and antibiotic sensitivity of biofilm-forming isolates were investigated on 104 K. pneumoniae and 100 E. coli isolates
Methods: Sample collection and identification of bacterial isolates was done. Antibiotic susceptibility testing was performed based on disc diffusion method. ESBL phenotypes were detected through the results of the antibiogram. MIC was measured using microtiter plate assay. Biofilm formation capability was accomplished. Total DNA was extracted. PCR was performed. Statistical analysis was done.
Results: Disc release sensitivity tests were performed according to Clinical Laboratory Standards Institute (CLSI) guidelines using discs containing gentamycin, amikacin, imipenem, tetracycline, ceftazidim, cefepime, ceftriaxone, cefotaxime, ciprofloxacin, and ampicilin. The highest level of resistance was against ampicilin (80% in E. coli and 96% in K. pneumoniae). 21 isolates of E. coli and 50 isolates of K. pneumoniae were able to form biofilm, which 7 isolates were able to form strong biofilm. In strong biofilm-forming isolates, the highest level of resistance was related to tetracycline (7% in E. coli and 7.2% in K. pneumoniae). 47 isolates of E. coli (47%) and 21 isolates of K. pneumoniae (20.2%) were classified as ESBL producers. 72 isolates from E. coli and 52 isolates from K. pneumoniae were classified as MDR.
Conclusion: Considering the role of biofilm in the transfer of genes, appropriate health policies and the correct administration of effective antibiotics can help in prevention.