مقالات پذیرفته شده در هفتمین کنگره بین المللی زیست پزشکی
POSSIBLE ANTIDIABETIC EFFECTS OF URTICA DIOICA HYDROALCOHOLIC EXTRACT AND MOLYBDENUM DISULFIDE NANOPARTICLES ON STREPTOZOCINE INDUCED PANCREATIC CELL LINE
POSSIBLE ANTIDIABETIC EFFECTS OF URTICA DIOICA HYDROALCOHOLIC EXTRACT AND MOLYBDENUM DISULFIDE NANOPARTICLES ON STREPTOZOCINE INDUCED PANCREATIC CELL LINE
Introduction: Diabetes Mellitus is known as a chronic metabolic disorder and it is characterized by hyperglycemia which could be caused by deficiency of insulin secretion, insulin action, or both.
Among various types of diabetes, type 1 DM (T1DM) and type 2 DM (T2DM) are the
most usually discussed. T1DM is also known as insulin-dependent diabetes. It is primarily due to pancreatic islet beta cell destruction and it leads to deficiency in insulin production in the body.
While T1DM cannot be prevented with current knowledge, T2DM can be prevented as well as treated easily, since management of diabetes is complex, it should include a healthy diet and lifestyle.
Nowadays, available therapies for diabetes include insulin and various oral antidiabetic agents, although they have beneficial roles in diabetes control and treatment, they cause unwanted side effects. On the other hand, in developing countries, as products are expensive and not easily accessible. So, using the traditional herbal medicines which are obtained from plants, plays an important role in the management of diabetes mellitus in some areas. Also, scientists show great interest in these plants because of their potential capacity to produce more efficient and safer medicine for diabetic patients.
One of the Medicinal Plants with a broad background in the treatment of diabetes is Urtica diouca (UD), which is known as nettle sting. On the other hand, nanotechnology has emerged as a new promising agent in the treatment of various diseases such as Alzheimer's and different types of cancers.
Methods: In this in vitro study we aimed to investigate the possible antidiabetic effects and Biocompatibility of molybdenum disulfide nanoparticles (MoS2 NPs), one of the less investigated two-dimensional metal nanomaterials, in living cellular environments. The pancreatic beta cell line (RIN-5F) was induced by streptozocine in order to intimate cell’s condition under diabetes stress. At first, the optimal concentration of UD hydro alcoholic extract and MoS2NPs were obtained by MTT test. Then, in the second phase of the study, insulin concentrations in the cell culture medium were measured by ELISA kit. The expression of some glucose metabolism-related genes like GCK, GLUT2, INS were also determined. In order to study the possible cytoprotective effect of the agents, BCl2 gene expression was measured too.
Results: According to our results, MoS2 NPs and UD hydroalcholic extract concentrations show increases in insulin secreting levels individually and synergic. And they show improved effects on some glucose metabolism-related genes like GCK,GLUT2,INS and apoptotic genes like BCL.
Conclusion: In conclusion, defined concentration of MoS2 NPs and UD hydroalcholic extract can show synergic cytoprotective effects and antidiabetic effects on some of the parameters but have antagonistic effects on others.