مقالات پذیرفته شده در هفتمین کنگره بین المللی زیست پزشکی
Delivery of CRISPR/Cas9 for antimicrobial resistance: Review Abstract Articles
Delivery of CRISPR/Cas9 for antimicrobial resistance: Review Abstract Articles
sadaf safaei,1,*Hamid Mir Mohammad Sadeghi,2
1. Department of pharmaceutical biotechnology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran 2. Department of pharmaceutical biotechnology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
Introduction: In last decades, antimicrobial drugs have been prescribed to inhibit the growth of bacteria, fungi and viruses. Development of antimicrobial resistance with sub-lethal concentration of antibiotic causes spread of acute and chronic infections. Therefore find the strategies for overcoming microbial resistance, is the key for the purpose of treatment of diseases.
CRISPR (Clustered Regularly Interspersed Short Palindromic Repeats)-Cas system is an adaptive immune system of bacteria and archaea. This system has regulatory effect on bacterial pathogenicity. The Cas9 nuclease of the CRISPR-Cas uses RNA-guided endonuclease provided the ability to rapidly and economically introduce sequence-specific modifications depends on the generation of double-strand break (DSB) and DNA repair process into the genomes of cell and organisms. Which means this sequence can be easily replaced by our desired sequence to retarget the CRISPR-cas9 nuclease and breaks antimicrobial resistance. Safe and efficient delivery of CRISPR/Cas9 systems is still a challenge. In this review, we discuss non-viral delivery systems based on nanoparticles for target delivery of CRISPR/Cas9 for Genome Editing.
Methods: CRISPR/Cas system is the most flexible and user-friendly platform for genome editing. Non-viral delivery systems based on nanoparticles are the most widely used method for target delivery of CRISPR/Cas9 for Genome Editing.
Results: Non-viral vectors, including Nano carriers and nanoparticles such as Nano polymeric- and lipid-based structures, rigid nanoparticles, nanoparticles coupled to specific ligand systems including arginine–glycine–aspartate (RGD) peptide, porous silicon, mesoporous silica, metal–organic, cell-penetrating peptides.
Conclusion: Non-viral vectors based on Nano carriers plays an important role for targeting delivery of CRISPR/Cas9 systems due to increase the circulation time, low toxicity, biocompatibility, and facilitating scaled up. The shape, size, and surface chemistry of NPs are the critical factors that regulate nuclear for cellular uptake, bio distribution, and rapid clearance.