مقالات پذیرفته شده در هفتمین کنگره بین المللی زیست پزشکی
The effects of L-serine on histopathological changes of pancreas in diabetic mice
The effects of L-serine on histopathological changes of pancreas in diabetic mice
Mahshad Sheikhi Narani,1Akram Vatannejad,2,*
1. Department of Comparative Biosciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran 2. Department of Comparative Biosciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Introduction: Diabetes mellitus is a common metabolic disorder as a world concern. It is caused by a lack of insulin in type 1 diabetes or a combination of insulin resistance and reduced insulin production in type 2 diabetes and gestational diabetes. Thus, there is a high need for therapeutic approaches to reduce the incidence of diabetes and its related complications. Growing evidence suggests a role for L-serine in the development of diabetes mellitus and its related complications, with L-serine being positively correlated to insulin secretion and sensitivity. The aim of this study is to investigate the possible pancreas histopathological changes caused by diabetes after treatment with L-Serine.
Methods: In order to carry out this research, 18 c57bl/6 male mice were purchased and divided into 3 groups (control, diabetic control and diabetic mice treated by L-serine(. Diabetes induced by streptozotocin, (200 mg/kg). After four weeks of oral administration of L-Serine (approximately 280 mg/day/mouse), animals were euthanized and pancreas tissue was used to determine pathological changes by H&E-stained techniques.
Results: In histopathological study of pancreas in the diabetic group, we discovered atrophy of the islets of Langerhans as well as reduction in their size and number. Abnormal histological changes significantly decreased in the diabetic group that supplemented by L-Serine.
Conclusion: These findings indicate that the supplement of L-Serine may has an effective influence on diabetes-induced pancreas tissue damage. These results are a foundation to further explore the use of L-serine supplement as a novel therapeutic intervention for pancreatic disorders in diabetes mellitus.