Introduction: Dealing with aging and reversing its process is always one of the
conditions that mankind is looking for a solution for.
In the last decade, in order to achieve this goal, research is done by
examining DNA and its methylation.
The study focused on physiological rejuvenation and analysis of specific and well-established epigenomic signatures of aging. The study suggests that chemical reprogramming may be used to treat blindness, liver failure, and skin damage. However, it is critical that the safety of chemical rejuvenation cocktails is tested rigorously in mammalian animal models before human trials are initiated.
Methods: For this research, various tests, including nuclear counterstaining with Hoechst 33342, wide field fluorescence imaging using the IXM-LZR, RNA sequencing and analysis using the Omega ENZA Total RNA kit, Agilent Tapestation, Illumina Novaseq, FastQC.
Genetic material has been used in some approaches to reverse aging, such as adeno-associated viral (AAV) delivery of DNA and lipid nanoparticle-mediated delivery of RNA. However, the delivery of genetic material can face potential barriers, including high costs and safety concerns associated with introducing genetic material into the body.
Results: Developing a chemical alternative to mimic the rejuvenating effects of genetic material could potentially delay aging and enable the treatment of various medical conditions.
Conclusion: chemically induced reprogramming to reverse cellular aging involves the use of a cocktail of small molecules, That includes various chemicals such as VPA, CHIR99021, Repsox-616452, Tranylcypromine, Forskolin,
Sodium Butyrate, bFGF, TTNPB, Y27632, SAG, ABT869, and α-KG. These chemicals work together to induce partial reprogramming of cells, which can reset the epigenome to a more youthful state and reverse cellular aging.