مقالات پذیرفته شده در هفتمین کنگره بین المللی زیست پزشکی
Effect of mitoquinone, resveratrol, and astaxanthin on the developmental potential of vitrified mammalian oocytes
Effect of mitoquinone, resveratrol, and astaxanthin on the developmental potential of vitrified mammalian oocytes
Sanaz Panahi Alanagh,1Fatemeh Hassani,2Azam Dalman,3Mahmood Talkhabi,4,*
1. Department of Animal Sciences and Marine Biology, Faculty of Life Sciences and Biotechnology, University of Shahid Beheshti, Tehran, Iran. 2. Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. 3. Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. 4. 1Department of Animal Sciences and Marine Biology, Faculty of Life Sciences and Biotechnology, University of Shahid Beheshti, Tehran, Iran.
Introduction: Oocyte vitrification is an established method for female fertility preservation during reproductive age. Vitrification increases reactive oxygen species (ROS), depletes ATP, and induces apoptosis in oocytes, all of which have a harmful effect on the mitochondrial membrane potential (ΔΨm) in oocytes. Antioxidants improve the developmental potential of the vitrified oocytes. It suggests that vitrification might require more advancement. This review will focus on antioxidants, such as astaxanthin(Ax), resveratrol, and mitoquinone (MitoQ).
Methods: In this review, the English keywords cryopreservation, oocyte, fertility, astaxanthin, resveratrol, and mitoqunine, as well as their Persian equivalents, were searched in the Persian database of Magiran as well as the English databases of Pubmed, Elsevier, Web of Sciences, and the Google Scholar search engine between the years 2010 and 2022. Numerous quantitative and qualitative studies that shared the same topics as the current study were chosen and assessed.
Results: 22 studies that were more closely relevant to the objective of the current study were chosen. The results of the review showed that MitoQ, RES, and Ax enhanced survival and ΔΨm of vitrified oocytes under treatment. They also demonstrated that the blastocysts from vitrified oocytes had higher mean cell counts when added antioxidants. By reducing the levels of ROS and raising the levels of glutathione (GSH), these measures lessened the oxidative stress of vitrified oocytes, reduced the incidence of abnormal meiotic spindles, and improved the aberrant mitochondrial distribution pattern. They significantly improved the survival rate of vitrified oocytes and enhanced the blastocyst production of fresh and vitrified oocytes. Furthermore, vitrified oocytes had a significantly increased relative fluorescence intensity of lysosomes, which the treatment was able to reduce. In vitrified oocytes, antioxidant treatment increased the mRNA levels of GDF9, SOD2, NRF2, and ATG5 in fresh and vitrified oocytes. The Bax/Bcl2 ratio and caspase3 mRNA expression was also considerably reduced in treated oocytes.
Conclusion: According to the results, using RES, Ax, and MitoQ may be useful for enhancinge the developmental potential of vitrification. This review showed that antioxidants might reduce cryopreservation damage during the vitrification of mammalian oocytes.