مقالات پذیرفته شده در هفتمین کنگره بین المللی زیست پزشکی
Investigating the effect of common mutations of SUGCT, TRPM8 and UFL1-AS1 genes in migraine patients in Tehran
Investigating the effect of common mutations of SUGCT, TRPM8 and UFL1-AS1 genes in migraine patients in Tehran
Maryam Rahmani,1,*Faranak Jamshidiyan,2Maryam Rouhani,3Majid Mesgar Tehrani,4
1. Department of genetic, East Tehran Branch, Islamic Azad University, Tehran, Iran 2. Department of genetic, East Tehran Branch, Islamic Azad University, Tehran, Iran 3. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran 4. Social Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Introduction: Migraine continues second among the world's causes of disability. Diagnosis is based on the history and clinical examination and imaging is usually not necessary. Migraine can be subdivided depending on whether there is an aura or not and based on the frequency of the headaches. The number of headache days determines whether the patient has episodic migraine or chronic migraine.
Most people who have migraines feel that people who do not have them often underestimate their condition. Migraines affect people's quality of life and ability to participate in work, family, and social events
Typical aura without headache is a known entity within the spectrum of migraine. Its pathophysiology is suggested to be similar to classic migraines, with cortical spreading depression leading to aura formation but without an associated headache. No clinical trials have been performed to evaluate treatment options, but case reports suggest that most patients will respond to the traditional treatments for migraine with aura. Bilateral greater occipital nerve blocks may be helpful in aborting migraine with prolonged aura.
Preventive therapy may also improve quality of life and prevent the progression to chronic migraines. Some indications for preventive therapy include four or more headaches a month, eight or more headache days a month, debilitating headaches, and medication-overuse headaches. Identifying and managing environmental, dietary, and behavioral triggers are useful strategies for preventing migraines.
Migraine is a complex brain disorder that is explained by the interaction of genetic and environmental factors. Migraine is the third leading cause of disability worldwide, leading to reduced quality of life and serious economic consequences Genome-wide association studies have identified several susceptibility variants that confer only modest increases in the global risk of migraine. Genetic studies have also shown the importance of common genetic factors between migraine and diseases such as depression and high blood pressure.
There are 4 types of migraine headaches: frontal, temporal, occipital and rhinogenic.. Migraine is a common neurovascular disorder that affects 10–20% of the world's population, commonly divided into migraine with aura (MA) and migraine without aura (MO). Several GWAS have been performed in migraine. In a GWAS, several million common DNA variants, so-called single nucleotide polymorphisms (SNPs) scattered throughout the genome, are tested in large groups for association with a trait. Our aim is to investigate the SNP in the common genes TRPM8, SUGCT, UFL1-AS1, which causes early diagnosis in people with migraine disease and helps to improve these people in the society.
Our subject by evaluating the occurrence of common mutations with the examined SNPs (13208321RS4379368, RS10166942, RS (genetic disease)
Methods: Using the PCR technique, The primary purpose of polymerase chain reaction (PCR) is to rapidly make many copies of a specific region of DNA or RNA so that it can be adequately identified, often by agarose gel electrophoresis. PCR is commonly used to amplify, modify and clone genes for expression studies. There are many uses for PCR, including paternity testing, biological relationships, mouse genotyping, diagnosing genetic diseases, forensics, and finding bacteria and viruses.
we check the SNP sequences of TRPM8, SUGCT and UFL1-AS1 genes ،Single nucleotide polymorphisms (SNPs) are DNA sequence changes that occur when a single nucleotide in the genome is different in paired chromosomes. Some SNPs change the amino acid sequence of a protein in the coding region, and others in the coding region. , do not affect the protein sequence. SNPs outside the coding region may also affect transcription factor binding, gene splicing, or mRNA degradation. With or without such effects on the biological function of gene products, SNPs serve as markers for investigating imbalances. Linkage and detection of genetic polymorphisms are very useful in population genetics research and medical sciencewith gene expression , and by observing the electrophoresis gel, we find out the effect of gene expression and mutation of these genes on migraine disease.
Results: This project helps us to inform the patient about the occurrence of mutations in the desired genes and the risk of developing migraines.
Conclusion: Our topic has not been evaluated by evaluating the common mutations with the investigated SNPs RS10166942, RS4379368, RS13208321, congenital genetic disease of migraine in Tehran, and changing the genotypic structure in the mutations that occurred and evaluating the phenotypic evidence in migraine patients depending on the social genotype of a country. it must be done.