• Therapeutic effects of D aspartate in a mouse model of multiple sclerosis
  • Sanaz afraei,1 seyed jalal zargar,2 Antimo D'Aniello,3,* Reza sedaghat,4 Gholamreza Azizi,5 Abbas Mirshafiey,6
    1. Department of Cell & Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran
    2. Department of Cell & Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran
    3. Laboratory of Neurobiology, Zoological Station of Naples “Anton Dohrn”, Villa Comunale, Napoli, Italy
    4. Departments of Anatomy and Pathology, Faculty of Medicine, Shahed University, Tehran, Iran
    5. Research Centre for Immunodeficiencies, Pediatrics Centre of Excellence, Children's Medical Centre, Tehran University of Medical Sciences, Tehran, Iran
    6. Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran


  • Introduction: Multiple sclerosis (MS) is an autoimmune disease of the central nervous system (CNS) that leads to an inflammatory demyelination and axonal damage. Experimental autoimmune encephalomyelitis (EAE) is experimental animal model for the study of MS. The purpose of the present investigation was to assay the therapeutic efficacy of D aspartic acid (D Asp) on a mouse EAE model.
  • Methods: Myelin 40 oligodendrocyte glycoprotein (35 55) in a complete Freund s adjuvant emulsion was used to induce EAE in female C57BL 6 mice, and D Asp was operated to test its efficiency in the reduction of EAE. Throughout the study period, clinical evaluation was assessed. On 21st day after induction EAE, blood samples were taken from the right ventricle of the heart for the evaluation of interleukin 6 and other chemical molecules. The mice were sacrificed, and their brain and cerebellum were removed for histological analysis.
  • Results: Our findings illustrated that D Asp had beneficial effects on EAE by reduction in the severity and delay in the onset of the disease. Histological analysis displayed that treatment with D Asp can decrease inflammation. In addition, in D Asp treated mice, the serum level of interleukin 6 was significantly lower than that in control animals, whereas the total antioxidant capacity was significantly higher.
  • Conclusion: The data represents that D Asp possess neuroprotective property, so it is able to prevent the onset of the multiple sclerosis.
  • Keywords: Experimental autoimmune encephalomyelitis, Multiple sclerosis, Antioxidant, D aspartic acid, D aspa