• The role of AGER in the P13K-AKT and JAK-STST signaling pathway and the direct relationship between decreased expression and decreasing mortality in lung cancer
  • Fariba Heidari Esfahani,1 Erfaneh Heidari Esfahani,2 Mohammad Rezaei,3 Mahdieh Bakhshayesh,4 Mansoureh Azadeh,5,*
    1. Zist Fanavari Novin Biotechnology Institute, Isfahan, Iran
    2. Zist Fanavari Novin Biotechnology Institute, Isfahan, Iran
    3. Zist Fanavari Novin Biotechnology Institute, Isfahan, Iran
    4. Zist Fanavari Novin Biotechnology Institute, Isfahan, Iran
    5. Zist Fanavari Novin Biotechnology Institute, Isfahan, Iran


  • Introduction: Lung cancer is the major cause of cancer mortality worldwide. The most common type of lung cancer is non-small cell lung cancer, which consists of three tissue subtypes LUAD, LUSC, and large cell lung carcinoma. The advanced glycosylation end product (AGE) receptor encoded by this gene is a member of the immunoglobulin superfamily of cell surface receptors [1][2][3][4] .
  • Methods: The GSE168466 dataset has been found in the GEO online database. Was examined the interaction between mRNA and lncRNA using lncRRIsearch. With miRWalk, we investigated miRNA mRNA interactions using all miRNAs recovered from DIANA-Tar Base v.8. Additional databases include KEGG, Reactome, and STRING. Comparing the expression of genes in lung cancer was explored by Gepia2 databases. The expression of lncRNAs in different tissues has been examined by the lnCAR databases. This result was also confirmed by GEPIA2 and ENCORI. Gene ontology was checked using the Enrich R database.
  • Results: AGER was selected as a significantly up-regulated gene (logFC=3.1, adj. Pvalue=7.81e-22) in tumor samples compared to normal samples from NSCLC Tissue. survival analysis showed a significant direct relationship between down expressed and decreasing mortality (Log-rank p=0.002 HR (high)=0.62 p(HR)=0.0023). According to the analysis, a potential ceRNA network between AGER, hsamiR-509-3P, and HELLPAR-001 can be established. The involvement of AGER in the DNA mismatch repair pathway can fortify the possibility of the importance of AGER in changing non-malignant cells into cancer cells and AGER plays an important role as a receptor in AGE-RAGE signaling pathway in diabetic complications with an effect on P13K-AKT signaling pathway which lead to Apoptosis as well as it can by JAK-STST signaling pathway impress on vascular remodeling.
  • Conclusion: We identified the hub lncRNA-mRNA network involved in regulating various biological processes from NSCLC Tissue. Interaction analysis of AGER, hsamiR-509-3P, and HELLPAR-001 illustrated to have a single local base-pairing interaction. in cervical cancer. Moreover, AGER by using the P13K-AKT signaling pathway causes Apoptosis and also with such an effect on the JAK-STST signaling pathway would be able to regulate vascular remodeling.
  • Keywords: Lung cancer (LC), JAK-STST signaling pathway, Apoptosis, P13K-AKT signaling pathway, ceRNA network