مقالات پذیرفته شده در هفتمین کنگره بین المللی زیست پزشکی
In-vitro evaluation of cytotoxic, anti-proliferative activity assessment of two tolerogenic probiotics on lung adenocarcinoma (A549 cell line)
In-vitro evaluation of cytotoxic, anti-proliferative activity assessment of two tolerogenic probiotics on lung adenocarcinoma (A549 cell line)
Ahmad Nejati Shahidain,1Akram Hoseinzadeh,2,*Mohammad Reza khakzad,3
1. Department of Biomedical engineering, Mashhad Branch, Islamic Azad University, Mashhad, Iran 2. Department of Immunology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran 3. Innovative Medical Research Center, Department of Immunology, Mashhad Branch, Islamic Azad
Introduction: Cancer is a fatal malignancy with high clinical significance and remains one of the major causes of illness and death. The safety and stability of the standard chemotherapeutics drugs and synthetic agents used to manage cancer are affecting the quality of life or contributing for development of drug resistance and are not affordable to the majority of the patients. Therefore, scientists are looking into clinical management of the cancer with high efficiency. Recently the potential impact of the probiotics on the disease has attracted the attention of researchers. Probiotics are useful and nonpathogenic microorganisms in the gastrointestinal tract which can show anticancer activity through the induction of apoptosis. Lactobacilli are a group of probiotics with beneficial effects on prevention of cancer by modulating the proliferation and maintaining homeostasis. Some studies suggest the administration of them efficiently serve as a reservoir in the treatment of a variety of different cancer diseases. The aim of this project was to evaluate the effects of Lactobacillus rhamnosus and Lactobacillus delbrueckii on proliferation of the A549 cell line.
Methods: The studied groups were divided and analyzed as follows (S) only A549 cell line like control group A549 cell line coculture with Lactobacillus rhamnosus A549 cell line coculture with Lactobacillus delbrueckii A549 cell line coculture with both probiotics. The perform analyzes at 48 hours after sowing in 96well plates. Individual groups were subjected to cytotoxicity and cell proliferation tests using MTT assay and within 48 hours. The data were submitted to statistical analysis by ANOVA with a significance level of 5 percent.
Results: This project in under study and the MTT result of the effects of our tolerogenic on A549 cell line will be presented in the congress.
Conclusion: Our results indicated that due to their apoptotic properties and reduction of proliferation on A549 cell line the use of these probiotics could probably represent a new tool for the better management of cancer. Further assessments are required to evaluate our results on the other cancer cell lines in advance to use these probiotics in other extensive trial studies.