مقالات پذیرفته شده در هفتمین کنگره بین المللی زیست پزشکی
Investigating the inhibitory effect of the fractions of extracellular secretions (Postbiotic) in Pediococcus probiotic bacteria on Gastrointestinal cancer
Investigating the inhibitory effect of the fractions of extracellular secretions (Postbiotic) in Pediococcus probiotic bacteria on Gastrointestinal cancer
Introduction: As per the 2020 report by the World Health Organization, cancer is the second most prevalent cause of death worldwide, causing almost 10 million deaths annually. Colorectal cancer accounts for a significant portion of the statistics, with approximately 2 million cases and 1 million fatalities. Nowadays a variety of studies have indicated that microorganisms function in the gastrointestinal have a vital role in the regulation of human homeostasis and the immune system. Therefore, microbiome manipulation and general metabolism of intestinal microflora are effective strategies for cancer treatment which includes probiotics and postbiotics. While probiotics are beneficial, postbiotics are considered to be a safer option with fewer side effects. Regarding postbiotics potential effectiveness of health as a promising and emerging method for cancer prevention and treatment, it is crucial to investigate the postbiotics as an anticancer.
Methods: The present study was employed postbiotic, derived from bacterial strains, to explore their inhibitory and anticancer effects on colon cancer cells. First of all, the strains of Pediococcus was isolated in the laboratory. Then, the cell-free supernatant (CFS) known as postbiotic was extracted, and provided through the lyophilization method. The cytotoxicity effect of the postbiotic sample was tested on the HCT-116 colon cancer cell line. The sample of postbiotic was prepared at concentrations of 50, 100, 250, 500, and 750 (nM), followed by MTT assay at 24, 48, and 72-hour intervals, and calculated IC50. The flow-cytometry was arranged by postbiotic concentration at 750 nM over a period of 48 hours to examine Apoptosis and Necrosis clles and the cell cycle arrest and ROS was analysed.
Results: The anticancer properties of postbiotic were studied based on the preparation of isolated strains. The determination of statistical significance was made with Two-way ANOVA. The MTT assay findings have clarified that postbiotic can able to induce cytotoxicity in HCT-116 cells, depending on concentration and time. There is a significant difference in the evaluated results of postbiotic with a concentration of 750 nM. The reduced cell survival result after 72 hours for cell-free supernatant of Pediococcus (CFSP) was obtained as 67%. According to the results, treatment of cancer cell lines with determined IC50 increased the percentage of cells in early and late apoptosis phases compared with the negative control. The percentage of induced apoptosis (early apoptosis) was 8.64%.
In comparison to the untreated control cells, treatment with CFSP at a concentration of 750 µg/ml for 48 hours led to an increase in the Sub-G1 phase of cells. CFSP treatment was associated with the highest sub-G1 phase of 62.61% when compared to the negative control.
The analysis of results reveals that the postbiotic possess notable anti-cancer properties.
Conclusion: The findings demonstrated that the lab-made postbiotic, the cell-free supernatant, might have inhibitory and lethal effects on colon cancer. It will be necessary to carry out further examinations and investigations to get more definitive results.