• A comparison between centrally and systemically administered erythropoietin on kidney protection in a model of fxed‑volume hemorrhagic shock in male rats
  • Mina Ranjbaran,1 Mehri Kadkhodaee,2 · Maryam Adelipour,3 · Leila Hafazeh,4 Keivan Lorian,5 Behjat Seif,6,*
    1. Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
    2. Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
    3. Department of Clinical Biochemistry, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
    4. Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
    5. Research and clinical center for infertility, Yazd Rreproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
    6. Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran


  • Introduction: In this study, a comparison between centrally and systemically administered erythropoietin (EPO) was performed on nephroprotection during hemorrhagic shock (HS) in mal
  • Methods: s Male rats were allocated into four experimental groups. (1) Sham; a guide cannula was inserted into the left lateral ventricle and other cannulas were placed into the left femoral artery and vein. (2) HS; stereotaxic surgery was done to insert a cannula in the left lateral ventricle and after a 7-day recovery; hemorrhagic shock and resuscitation were performed. (3) EPO-systemic; the procedure was the same as the HS group except that animals received 300 IU/kg erythropoietin into the femoral vein immediately before resuscitation. (4) EPO-central; animals was treated with erythropoietin (2 IU/rat) into the left lateral ventricle before resuscitation. Arterial oxygen saturation (SaO2) was measured during experiments. Urine and renal tissue samples were stored for ex-vivo indices assessments.
  • Results: Erythropoietin (systemically/centrally administered) signifcantly improved SaO2, renal functional and oxidative stress parameters and decreased renal infammatory (TNF-α and IL-6) mRNA expression compared to the HS group. EPO-treated groups showed a decrease in active form of caspase-3 protein level and an increase in autophagy activity in comparison with the HS group.
  • Conclusion: Considering the fact that the efective dose of systemic EPO (300 IU/kg) was roughly 50 times higher than that of central administration (2 IU/rat), centrally administered EPO was accompanied by more advantageous consequences than systemic way. EPO is likely to act as a neuro-modulator or neuro-mediator in the central protection of organs including the kidneys
  • Keywords: Erythropoietin · Hemorrhagic shock · Inflammation · Intracerebroventricular infusion · Stereotaxic