مقالات پذیرفته شده در هفتمین کنگره بین المللی زیست پزشکی
Assessment of Antioxidant Properties of PDA-Coated Curcumin Nanoparticles: A Serial Dilution Study
Assessment of Antioxidant Properties of PDA-Coated Curcumin Nanoparticles: A Serial Dilution Study
Mahshad Hajia,1Mina Bordbar,2Dr Ali Khatibi,3,*
1. department of biotechnology, faculty of biological sciences, Alzahra university 2. department of biotechnology, faculty of biological sciences, Alzahra university 3. department of biotechnology, faculty of biological sciences, Alzahra university
Introduction: Oxidative stress-induced reactive oxygen species (ROS) play a critical role in various diseases, making antioxidants essential in maintaining cellular homeostasis. Curcumin, a natural polyphenolic compound with potent antioxidant properties, has been widely studied for its potential health benefits. However, its limited bioavailability hinders its therapeutic efficacy. To address this challenge, we synthesized polydopamine (PDA)-coated curcumin nanoparticles and assessed their antioxidant effects at various concentrations. We hypothesized that the antioxidant potential of these nanoparticles would increase with higher concentrations.
Methods: PDA-coated curcumin nanoparticles were synthesized and characterized at 25°C. Six serial dilutions (1/2 dilution each) were prepared using water, resulting in a range of nanoparticle concentrations. The antioxidant properties of each sample were evaluated using two distinct methods: UV-Vis spectrometry and the Fenton reaction, involving the generation of ROS by Fe2(SO4)3 and their subsequent scavenging by salicylic acid. ROS clearance was quantified for each concentration.
Results: Our results demonstrated a clear concentration-dependent increase in the antioxidant properties of PDA-coated curcumin nanoparticles. UV-Vis spectrometry revealed a gradual rise in ROS scavenging capacity as the nanoparticle concentration increased. The ROS clearance values for the six concentrations were 0.45, 0.33, 0.31, 0.23, 0.22, and 0.21, respectively. This trend was further validated using the Fenton reaction method, which consistently showed enhanced ROS scavenging with higher nanoparticle concentrations. The observed dose-dependent effect supports our hypothesis that the antioxidant potential of PDA-coated curcumin nanoparticles is positively correlated with their concentration.
Conclusion: In this study, we successfully synthesized PDA-coated curcumin nanoparticles and demonstrated their increased antioxidant properties with rising concentrations. These findings suggest the potential utility of these nanoparticles as an efficient antioxidant delivery system. The ability to enhance the bioavailability and antioxidant activity of curcumin through nanoparticle formulation holds promise for various therapeutic applications, particularly in combating oxidative stress-related diseases. Further research is warranted to explore the translational potential of these nanoparticles in vivo and their specific mechanisms of action.