مقالات پذیرفته شده در هفتمین کنگره بین المللی زیست پزشکی
Assesment of Gut Bacterial Profile in Diabetic Patients Undergoing Bariatric Surgeries Using Quantitative PCR”
Assesment of Gut Bacterial Profile in Diabetic Patients Undergoing Bariatric Surgeries Using Quantitative PCR”
Mahnaz Dabagh,1sara minaeian,2,*soheil rahmani fard,3fatemeh faraji,4milad sabaee,5Abdolreza Pazouki,6
1. Department of Microbiology, School of Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran 2. Antimicrobial Resistance Research Center ,Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences,Tehran, Iran. 3. Antimicrobial Resistance Research Center ,Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences,Tehran, Iran. 4. Antimicrobial Resistance Research Center ,Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences,Tehran, Iran 5. Antimicrobial Resistance Research Center ,Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences,Tehran, Iran 6. Antimicrobial Resistance Research Center ,Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences,Tehran, Iran
Introduction: Introduction: : In recent years, studies regarding the association of gut microbiome and multifactorial genetic disorders such as diabetes, has gained a lot of traction. Furthermore, metabolites excreted from gut microbiota have broad effects on genes responsible for a wide range of human disorders. The aim of this study was to evaluate changes in gut bacterial load in obese diabetic patients undergoing bariatric surgery.
Methods: Methods: Thirty obese and eligible patients were recruited from the Obesity Clinic of Rasool-E-Akram Hospital Complex in Tehran, Iran between May 2021 to May 2022. Blood and stool samples were collected from patients at two time-points: (1) before surgery (2) 6-months after surgery. These samples were used to determine the effect of the surgery on metabolic indices and gut microbiota via quantitative PCR.
Results: Results: Bariatric surgery showed tremendous potential for alleviating the metabolic imbalance. Analyzing patient subgroups revealed that these surgeries can effectively alter the level and ratio of gut bacteria in diabetic patients with habit of volume eating. In these patients the relative proportion of Bifidobacter to Lactobacillus and Bifidobacterium to total load were decreased, while proportions of Bacteriodetes to Bifidobacterium and Provetella to Bifidobacterium were significantly increased.
Conclusion: Conclusion: Gathered results regarding changes in gut microbiota profile of diabetic patients and alleviation of the existing dysbiosis can be used as a potential therapeutic approach in treatment.