• Fabrication and characteristics of chitosan nanofibrous mat coated by hyaluronic acid and resveratrol as a tissue engineering scaffold
  • Asma Moghaddam,1 Mahmoud Orazizadeh,2,* Fereshteh Nejaddehbashi,3 vahid Bayati,4 Mahmoud Zardast,5 Mohammadreza Abbaspour,6
    1. Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
    2. Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
    3. Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
    4. Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
    5. Department of Pathology, Birjand University of Medical Sciences, Birjand, Iran
    6. Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran


  • Introduction: The healing of wounded skin possesses intricate nature. Fabricating of scaffolds as wound dressing has been noticed in clinical applications. In the present study, we designed nanofiber mats of chitosan (CS) via electrospinning with subsequent coating by Hyaluronic acid (HA) and Resveratrol (RS) for their efficacy in immunoprotection and pro-angiogenic features respectively.
  • Methods: The developed nanofibrous mats were characterized by SEM, FTIR, TGA, tensile testing, in vitro release study and human dermal fibroblasts seeding assay.
  • Results: The results revealed that human dermal fibroblasts adhered and proliferated in all scaffolds and no significant cytotoxicity was observed. However, cells infiltrated deep into the scaffold were more detected in porous CS/HA and CS/HA/RS scaffolds compared to the CS scaffold. CS/HA nanofibers indicated more interactions between HA and CS. Water absorption measurement revealed differences in the wettability of resultant fibers. Although both scaffolds showed high wettability, the CS/HA/RS had lower wettability than CS/HA.
  • Conclusion: The present findings indicate that the fabricated electrospun CS/HA and CS/HA/RS scaffolds has excellent biomechanical properties and may be a preferable candidate scaffold, since it can also effectively serve as a carrier of growth factors because of its structural, mechanical, and releasing properties.
  • Keywords: Chitosan, Hyaluronic acid, Electrospinning, Resveratrol, Nanofiber