• Iranian Journal of Blood & Cancer
  • Narges Mardani,1,* Amin Soltanpor Dehkordi,2 Maryam Sayahinouri,3 Haniyeh Sadat Hosseininia,4 Arman Saadati Partan,5 Arasalan Jalili,6
    2. Department of Mycology and Parasitology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, IR Iran
    3. Department of Immunology, Afzalipour Faculty of Medicine, Kerman University of Medical Sciences, Kerman, IR Iran
    4. Department of Cellular and Molecular Biology, Faculty of Advanced Medical Science, Islamic Azad University of Medical Sciences, Tehran, IR Iran
    5. Parvaz Research Ideas Supporter Institute
    6. Parvaz Research Ideas Supporter Institute


  • Introduction: According to the lastest WHO report, lung cancer ranks among the top cancer- associated mortalities. Morever, it has been related to a high rate of metastasis, which indicates the importance of Angiogenesis. Histologically, lung cancer is divided inti NSCLC and SCLC, with NSCLC being the most common. Angiogenesis is essential for tumor development. Additionally, immune cells, soluble factors, and ECM play a crucial role in their formation. this study reviews the angiogenesis formation factors in previous studies as well as analyzes in silico angiogenesis-related genes in NSCLCs.
  • Methods: First, three high-throughput GEO data sets with 18 lung cancer and normal samples were adopted to achieve the study purpose. Then, the up-and-down-regulated genes with p-value < 0.05 were isolated. Next, the genes were taken to the Enrichr and the KEGG databases. Lately, our in-silico analysis confirmed the gene expression connection between angiogenesis and lung cancer invasion.
  • Results: It is reported that EPhB2, PIK3R2, HSPB1 and Wnt7b is the most prevalent in NSCLC subtypes. Moreover, a decrease of 50% in overall survival in both low and high Wnt7b transcripts per million was observed.
  • Conclusion: In conclusion, the gene expression association between the development of angiogenesis and lung cancer invasion has been proven by in silico analysis. The most upregulated genes which showed high logfc in lung cancer in comparison with the healthy patient have been identified. Among them, Wnt7b, due to its high logfc, distinct regulation, and carcinogenesis, was chosen as the candidate. Furthermore, in silico analysis highlights Wnt7b's importance in tumor cells. This regulates angiogenesis, and carcinogenesis, and is critical for normal lung tissue development. The violin plot (As shown in Figure 4) and overall survival graph (As shown in Figure 5) have demonstrated that the distinct role of Wnt7b in lung cancer needs more consideration. Our result has fully illuminated the significance of angiogenesis-related genes in lung cancer progression. In particular, Wnt7b plays a crucial role in regulating survival rates. In silico our analysis has shed light on the potential of Wnt7b in enhancing the overall survival rate. Finally, future in vitro and in vivo studies should provide a more reliable understanding of its regulatory function.
  • Keywords: Wn7tb EPhB2 Angiogenesis Lung cancer In silico Bioinformatics