• Detection of virulence Genes of Escherichia coli O157:H7 strains from Urinary tract infections in Isfahan
  • Parisa behshood,1,* Fateme Talebi varnosfaderani,2 Elahe Tajbakhsh,3
    1. PhD, Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
    2. PhD, Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
    3. PhD, Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran


  • Introduction: E. coli O157:H7 is one of the main factors that lead to diseases such as diarrhea, bleeding colitis, uremic hemolytic syndrome, and even death in men. This study aimed to determine and survey the prevalence of Escherichia coli O157:H7 virulence Genes from Urinary tract infections in Isfahan
  • Methods: In this study 100 urine samples were collected from nosocomial infection during 6 months. Then, isolates were identified with specific tests. Then isolation of E.coli O157:H7 was confirmed with the use of specific antisera and the presence of E.coli O157:H7 virulence genes including stx1, stx2, eaeA was analyzed with multiplex PCR method
  • Results: From50 strains of E.coli O157:H7 isolated,20 % of the isolates with genes stx2 and eaeA. the stx1 gene was in 10 % of the isolate. and no isolates were observed with all three genes.
  • Conclusion: E.coli O157:H7 strain is an important public health concern. Preventive measures may reduce the number of who carry it.
  • Keywords: E.coli , urinary tract infections, multiplex PCR