مقالات پذیرفته شده در هفتمین کنگره بین المللی زیست پزشکی
Combination effect of Cis-platin and selenium nanoparticles on cytotoxicity and apoptotsis against colon cancer (HT29) cell line and evaluation of caspase 8 and 9 genes expression
Combination effect of Cis-platin and selenium nanoparticles on cytotoxicity and apoptotsis against colon cancer (HT29) cell line and evaluation of caspase 8 and 9 genes expression
Introduction: The use of chemotherapy drugs, along with its benefits, can have severe side effects and also cause drug resistance. One of the ways to reduce the required dose of chemotherapy drugs is to use combined treatments. The aim of this research was to evaluate the combined treatment of cisplatin and selenium nanoparticles against HT-29 colon cancer cells.
Methods: HT-29 cell line was obtained from Tehran Pasteur Institute cell bank and cultured in RPMI1640 medium. Cells were treated with different concentrations of cisplatin, selenium nanoparticles and the combination of both and their survival was evaluated after 24 hours using MTT test. Also, the expression of caspase 8 and 9 genes in the studied cells under the combined treatment of selenium nanoparticles and cisplatin was investigated using real time PCR method. Finally, the percentage of apoptosis in the cells treated with the mentioned compounds was measured using the flow cytometry method and the Annexin/PI kit. The obtained data were analyzed using SPSS software.
Results: The results showed that the IC50 values for cisplatin, selenium nanoparticles, and the combination of cisplatin and selenium nanoparticles were 51, 75 , and 2.9 μg/ml, respectively, which indicates that their combination is more cytotoxic against HT-29 cancer cells. On the other hand, the expression of caspase 8 and 9 genes under the influence of selenium and cisplatin nanoparticles was significantly increased in treated cells (P<0.05). Flow cytometry evaluation showed that the number of apoptotic cells increases significantly after the combined treatment of selenium nanoparticles and cisplatin. It was observed that cells with early apoptosis increased by 25.04% and cells with delayed apoptosis by 19.10%.
Conclusion: The results of the present research show that selenium nanoparticles can be used alongside the anticancer drug, cisplatin, and in addition to reducing the required dose of the drug, it increases the efficiency of the treatment and induces apoptosis in HT-29 cancer cells.