مقالات پذیرفته شده در هفتمین کنگره بین المللی زیست پزشکی
Detection of PI3K gene expression difference and clinicopathology of samples in paraffin block tissues of gastric adenocarcinoma
Detection of PI3K gene expression difference and clinicopathology of samples in paraffin block tissues of gastric adenocarcinoma
Dr.Faramak Jamshidian,1Dr.Shohre Zare,2,*
1. Department of Biology , East Tehran Branch , Islamic Azad University , Tehran , Iran 2. Department of Genetics and Biology , School of Biological Science , Varamin-Pishva Branch , Islamic Azad University , Varamin , Iran
Introduction: Gastric cancer is a global health problem, with more than 1 million people newly diagnosed with gastric cancer worldwide each year. Despite its worldwide decline in incidence and mortality over the past 5 decades, gastric cancer remains the third leading cause of cancer-related death. Knowledge of global as well as regional epidemiology and risk factors for gastric cancer is essential for the practicing gastroenterologist to make personalized decisions about risk stratification, screening, and prevention. In this article, we will examine the expression of PI3K gene and it`s regulators and compare this expression difference between tumor and healthy tissue.
Methods: In this study, after collecting samples, RNA extraction and cDNA synthesis were performed. Then, we used the Real-time PCR technique to check the gene expression, and the data obtained from it were analyzed through Rest2009 software.
Results: The results of the data analysis showed that the expression of PI3K gene increased in tumor tissues compared to healthy ones, and this expression difference between healthy and tumor tissue is significant.
Conclusion: The significant expression difference of PI3K and the obtained data show that this gene can be introduced as a gastric cancer biomarker. More research should be done to be sure.