مقالات پذیرفته شده در هفتمین کنگره بین المللی زیست پزشکی
The apoptotic effects of myricetin on Breast Cancer Cells by induction of extrinsic and intrinsic apoptotic pathways
The apoptotic effects of myricetin on Breast Cancer Cells by induction of extrinsic and intrinsic apoptotic pathways
mansour homayoun,1Nayereh Sajedi,2mehrnoush malekzadeh,3Mitra Soleimani,4,*
1. Department of Anatomical Sciences, Isfahan University of Medical Sciences, Iran. 2. Department of Anatomical Sciences, Isfahan University of Medical Sciences, Iran. 3. Department of Anatomical Sciences, Isfahan University of Medical Sciences, Iran. 4. Department of Anatomical Sciences, Isfahan University of Medical Sciences, Iran.
Introduction: Myricetin is a polyphenol flavonoid with nutraceutical values which is abundantly found as the main
ingredient of various foods and beverages. It has been reported that the function of myricetin is to trigger apoptosis in several types of cancers. The present study intended to investigate the apoptotic effects of myricetin on MCF-7 breast cancer cells and to assess its possible mechanisms of action.
Methods: MCF-7 breast cancer cells were assigned to four groups: Control (cells in normal condition); myricetin (cells treated with the IC50 dosage of myricetin) in three different incubation times (24, 48, and 72 h). The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, annexin V assay, flow cytometry, real-time polymerase chain reaction (PCR), and caspase-3 assay were used to estimate the apoptosis function of myricetin in breast cancer.
Results: The expression levels of apoptosis-related
genes caspase-3, caspase-8, caspase-9, and the BAX /Bcl-2 ratio as well as the expression of p53, BRCA1, GADD45
genes were significantly increased following the treatment of MCF-7 breast cancer cells with myricetin. The annexin V assay demonstrated the significant expression of annexin which was also detected by flow cytometry.
Conclusion: Myricetin efficiently induces apoptosis in MCF-7 breast cancer cells by evoking both extrinsic and intrinsic apoptotic pathways. Myricetin may exert its apoptotic effects on MCF-7 cells by inducing the BRCA1- GADD45 pathway.