Introduction: Treatment with limbus transplantation has been developed for superficial eye diseases and damage that causes limbus stem cell defects, and eye epithelial stem cells can improve the surface and internal function of the eye as well as the cornea with natural lenses.
Methods: Epithelial cell and stem cell culture method was used by heat shock proteins and after culture, the cells should be prepared from the separated culture medium for transplantation and in fact amniotic membrane with extracellular matrix elements such as fibronectin-laminin-collagen IV and growth factors EGF, bFGF, HGF are effective in maintaining stemness properties in limbal epithelium stem cells.
And it was transplanted with a natural lens on the cornea of the patient's eye. If both stem cell transplants of the limbus area and cornea transplant were needed, these operations were performed simultaneously or sequentially.
Results: The results of this test indicate that when the epithelial cells were cultured in the processed medium and transplanted with the cornea by a natural lens, the internal inflammations were reduced and the patient was able to get rid of his annoying eye diseases.
Conclusion: Dental pulp stem cells, hair follicles, and bone marrow mesenchymal cells are among the investigated sources, and in animal models, the possibility of using these cells and their ability to form corneal epithelial cells has been confirmed, and by conducting additional tests and confirming the results In human models, they will probably replace today's common methods in the near future.