مقالات پذیرفته شده در هفتمین کنگره بین المللی زیست پزشکی
Reparative effects of local and intraperitoneal application of platelet-rich plasma (PRP) in hypothalamic arcuate nucleus lesion model in female rats.
Reparative effects of local and intraperitoneal application of platelet-rich plasma (PRP) in hypothalamic arcuate nucleus lesion model in female rats.
Elham Abbasi,1,*Morteza Behnam Rasouli,2Ali Moghimi,3zeinab Neshati,4
1. department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran 2. department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran 3. department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
Introduction: In female rats, chemical destruction of the arcuate nucleus causes the cessation of sexual cycles (estrous cycle). In this regard, if the arcuate nucleus is reconstructed and repaired, it is possible to re-establish the estrus cycles. In this research, the restorative or protective effects of PRP on the neurons of the arcuate nucleus were investigated in several different time periods after the destruction of the arcuate nucleus.
Methods: 90 female Wistar rats were divided into 8 experimental groups (n=10) and a control group. The experimental groups included the negative control (bilateral destruction of the arcuate nucleus with quinolinic acid) and the groups receiving local PRP included immediately, 24h, 48h, 72h, (immediately and 24h), (immediately and 24h and 48h), (immediately and 24h and 48h and (They were divided 72h after creating a bilateral lesion in the arcuate nucleus. Then, after 2.5 months of daily smearing, the mice were deeply anesthetized and the brain was removed for RT PCR and brain and ovary for histological studies.
Results: Creating a bilateral lesion of the arcuate nucleus with quinolinic acid causes the cessation or disruption of the estrus cycle, and the administration of PRP protects or restores the arcuate nucleus. The time of post-injury administration and the number of administrations are important in the effects of PRP, and the earlier the application of PRP, the more evident its reparative effects. Counting the neuronal density in the arcuate nucleus and ovarian weight and counting the primary ovarian follicles in the groups receiving local PRP showed a significant difference from each other (P<0.001)), a significant decrease compared to the healthy control group (P<0.01) and a significant increase with the negative control group (p<0.01). The relative mRNA expression of case peptin, neurokinin B and dynorphin in the groups receiving local PRP is significantly different from each other (P<0.001), a significant decrease compared to the healthy control group (P<0.01) and a significant increase compared to the negative control group (p< 0.01).
Conclusion: The arcuate nucleus was used as a model to evaluate the ability of PRP in repairing nerve lesions or preventing the exacerbation of nerve lesions. Cessation of the estrous cycle and its resumption was considered as a measure to evaluate the restorative or protective effects of PRP. In this regard, the effects of the time factor after applying the lesion in the application of PRP as well as the number of times of its application were considered.