Introduction: Therostochytrids are aerobic and marine heterotrophic microorganisms that include genera such as Therostochytrium, Schizochytrium, Japonochytrium, and Aurantochytrium. Aurantochytrium is a single-cell marine oleaginous microorganism that is able to accumulate a large amount of omega-3 fatty acids and docosahexaenoic acid. These oils are a sustainable substitute for fish oil. Docosahexaenoic acid fatty acid prevents the occurrence of cardiovascular diseases and contributes to the proper functioning of the brain and eye health. Chronic inflammation is a risk factor for cancer, and the consumption of omega-3 fats such as docosahexaenoic acid, which has anti-inflammatory effects, reduces the risk of developing cancer, including colon, pancreatic, breast, and prostate cancer. Also, docosahexaenoic acid can have the benefits of chemotherapy. It improves treatment and cellular studies show that it may even inhibit the growth of cancer cells.
Methods: Optimizing the culture medium of Aurantochytrium strain was done with laboratory adaptive evolution technology and then the amount of cell dry weight, oil and docosahexaenoic acid was calculated. By using this technology, the cells are adapted to the selected conditions in the culture environment and during successive generations, and their growth and production are increased. The parameters considered in carrying out laboratory adaptive evolution technology include the effective Faulknor of glucose concentration, factor sensitivity test, determining the concentration or amount of selective pressure, selecting the final level of selective pressure or two higher levels, and finding the species adapted to stress conditions. In the optimization of the culture medium of Aurantochytrium strain, glucose was measured in six concentrations of 1%, 2%, 3%, 5%, 7%, 10% in the medium containing artificial seawater, yeast extract, peptone water.
Results: By calculating the cell dry weight of the concentration The optimal glucose used was %10.
Conclusion: The results showed that during ten consecutive generations, the amount of biomass and oil in ALE 10 strain increased compared to the parent strain.