Introduction: The gut microbiota has been shown to play a crucial role in various physiological processes, including digestion, metabolism, and immune function. Recent studies have also suggested that the gut microbiota can influence the nervous system, including behavior, mood, and cognitive function. However, the mechanisms underlying these effects are not fully understood. In this study, we aimed to investigate the effects of gut microbiota on the nervous system using a mouse model.
Methods: Thirty male mice were randomly assigned to two groups: a control group and a group treated with antibiotics to deplete gut microbiota. After four weeks of treatment, the mice underwent a battery of behavioral tests, including the open field test, elevated plus maze test, and Morris water maze test. The mice were then sacrificed, and their brains were collected for histological analysis.
Results: The results showed that the depletion of gut microbiota led to significant changes in behavior, including increased anxiety-like behavior and impaired spatial learning and memory. Histological analysis also revealed alterations in the structure and function of the hippocampus, a brain region critical for learning and memory.
Conclusion: Our findings suggest that gut microbiota plays a crucial role in the regulation of the nervous system, particularly in behavior and cognitive function. Further studies are needed to elucidate the underlying mechanisms and potential therapeutic implications of these findings.