Introduction: Gastric cancer is recognized as the fourth most common cancer in the world. Several factors are involved in gastric cancer, including suppression of apoptotic initiator genes (caspase-8 and caspase-9). This study aimed to evaluate the expression of caspase 8 and 9 genes in healthy and healthy individuals.
Methods: In this study, 30 patients with gastric cancer and 30 healthy individuals from the general population were selected as the control group. After blood sampling, RNA was extracted using the yekta tajhiz azma kit and the cDNA was prepared using the Taccara kit. Expression of Casp8 and Casp9 genes was then evaluated using Real-Time RT-PCR. Data were analyzed using Rest software.
Results: According to the obtained data, statistical analysis was performed (p <0.05) by Rest software, the expression of Casp8 and Casp9 in cancers was significantly decreased compared to healthy ones. (pCasp8 = 0.0022) and (pCasp9 = 0.0092). This finding indicates a decrease in the expression of this gene in gastric cancer.
Conclusion: Due to the pre-apoptotic role of Casp8 and Casp9 within the cells, it can be concluded that this gene is suppressed and the cells progressed to the tumor in gastric cancer specimens. The two genes also can be a good marker for cancer detection and prevention of the occurrence of cancer.