Introduction: Mitogen-activated protein kinase3 (MAPK3) kinases are proteins that are specific for the amino acids serine, threonine, and tyrosine. MAPKs belong to the CMGC kinase group (CDK/MAPK/GSK3/CLK). MAPKs are involved in directing cellular responses to a diverse array of stimuli, such as mitogens, osmotic stress, heat shock, and proinflammatory cytokines. They regulate cell functions including proliferation, gene expression, differentiation, differentiation, cell survival and apoptosis. This study aims to analyze the bioinformatic characteristics of this disorder and evaluate the stability of this gene.
Methods: The characteristics of this gene were checked by GC counter and protparam sites.
Results: The characteristics of this gene were checked by GC counter and protparam sites. The results of the study showed that the percentage of GC was 35.5%. The theoretical pI of the investigated protein was 5.61. In this range, the target protein is precipitated. The higher the GC percentage of the examined protein, the more stable that protein is. Also, the instability index and aliphatic index for MAPK3 in the studied 36.92 and 89.89, respectively. Average GRAVY (hydrophobic property of protein) calculated for proteins is obtained by dividing the sum of hydropathy calculated for all amino acids in the protein by the total number of amino acids of that protein. In this research, Grand average of hydropathicity (GRAVY) -0.349 was obtained, which indicates the non-polarity of the investigated protein.
Conclusion: The results of bioinformatic analysis showed that this protein is the stable proteins and is able to maintain its structure completely at high temperatures.