Secretions Released From Menstrual Blood Derived Stem Cells Facilitate Spermatogenesis Restoration in Busulfan-Induced Non-obstructive Azoospermic Mice
Secretions Released From Menstrual Blood Derived Stem Cells Facilitate Spermatogenesis Restoration in Busulfan-Induced Non-obstructive Azoospermic Mice
Mohammadmehdi Barfar,1Seyedeh Zeynab Peyghambarzadeh,2Hannaneh Golshahi,3,*
1. Department of Veterinary Medicine, Shoushtar branch, Islamic Azad university, Shoushtar, Iran 2. Assistant professor in Clinical Pathology, Department of Veterinary Medicine, Shoushtar branch, Islamic Azad university, Shoushtar, Iran. 3. Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Introduction: Nonobstructive azoospermia (NOA) is one of the most severe forms of male infertility, with limited treatment options. The aim of this study was to investigate whether the administration of Menstrual blood-derived stem cell secretome could promote spermatogenesis restoration in the busulfan-induced NOA mice model.
Methods: 18 adult mice were divided into three experimental groups: (1) Sham, (2) no treated group (NOA model), and (3) NOA+secretome administration. Secretome was obtained from menstrual blood mesenchymal stem cells (MenSCs). Ten microliters of secretome were first injected into the rate testis space in mice with NOA. Then, they received 500 microliters once a week for eight consecutive weeks intraperitoneally. Afterward, the animals were euthanized, and testis samples were taken for further evaluation.
Results: The spermatogenesis recovery was seen in secretome-administered groups in NOA mice that were confirmed by semen analysis and histopathological studies. Furthermore, the results showed that expression of DAZL, Vasa, Stra8, and Sycp3 was significantly increased in the secretome received group compared with the non-treated group, as demonstrated using quantitative real-time polymerase chain reaction.
Conclusion: In summary, our results indicated that the secretome of MenSCs could significantly facilitate spermatogenesis restoration of busulfan-induced NOA mice through paracrine activities.