Co-culture of SHED-MSCs and inflammatory M1 macrophages alters the TAC and MDA levels as an oxidative stress index
Co-culture of SHED-MSCs and inflammatory M1 macrophages alters the TAC and MDA levels as an oxidative stress index
Ali Fallah,1Azadeh Mohammad-Hasani,2Ayyoob Khosravi,3,*Abasalt Hosseinzadeh Colagar,4Mohsen Saeidi,5
1. Department of Molecular and Cell Biology, Faculty of Basic Science, University of Mazandaran, Babolsar, Iran. 2. Department of Molecular Medicine, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran 3. Department of Molecular Medicine, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran 4. Department of Molecular and Cell Biology, Faculty of Basic Science, University of Mazandaran, Babolsar, Iran. 5. Department of Immunology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran
Introduction: In inflammatory situations, SHED-MSCs exhibit properties that can modulate the immune response. These cells interact with various types of immune cells, including naïve or M1 macrophages. We believe that when SHED-MSCs are co-cultured with M1 macrophages, it may have an impact on oxidative stress markers such as total antioxidant capacity (TAC) and malondialdehyde (MDA), a peroxidation product. To examine the connection between TAC and MDA as biomarkers for oxidative stress in the supernatant of co-cultured SHED-MSCs and M1 macrophage cells.
Methods: The differentiation of the THP-1 monocyte cell line resulted in the formation of M1 macrophage cells. Once confirmed through flow cytometry, these cells were co-cultured with SHED-MSCs in a trans-well system. The levels of TAC and MDA in the supernatant were then measured using FRAP and TBARS methods, respectively.
Results: In the co-cultures supernatant, the average TAC levels in M1/SHED-MSCs were significantly higher compared to the control group. On the other hand, the average MDA concentrations in the co-cultures supernatant were lower compared to those of controls.
Conclusion: SHED-MSCs have the ability to alter oxidative stress indicators through their interaction with M1 macrophages. This indicates that SHED-MSCs have the ability to alter inflammation markers and modulate the inflammatory condition.