Co-culture of SHED-MSCs with M0 macrophages changes oxidative stress levels
Co-culture of SHED-MSCs with M0 macrophages changes oxidative stress levels
Azadeh Mohammad-Hasani,1Ali Fallah,2Ayyoob Khosravi,3,*Mohsen Saeidi,4Saeed Mohammadi,5
1. Department of Molecular Medicine, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran, Post Code: 4918936316, Iran 2. Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar 3. Department of Molecular Medicine, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan 4. Department of Immunology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran 5. Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran, Stem Cell Research Center, Golestan University of Medical Sciences, Gorgan, Iran
Introduction: SHED-MSCs modulate the immune response by interacting with many immune cells, such as naïve or M0 macrophages. Since M0 macrophages can play an important role in inflammation and can modify important components of the microenvironment, we hypothesized that the co-culture of SHED-MSCs and M0 macrophages could increase antioxidant capacity (TAC) and reduce peroxide products such as malondialdehyde (MDA) as indices of oxidative stress and sensitive markers of inflammation.
Methods: The effect of SHED-MSCs was evaluated by co-culture with THP-1-derived M0 cells, TAC levels by FRAP analysis, and MDA levels by TBAR methods in supernatant co-culture.
Results: Mean TAC levels in the co-culture supernatant were found to be significantly higher compared to the control supernatant mean. The mean levels of MDA were significantly reduced in the co-cultured supernatant compared to the mean of the control or untreated supernatant.
Conclusion: As suggested by our study, SHED-MSCs have the ability to alter indices of oxidative stress and modulate inflammatory conditions