• hAMSCs secretome enables to regulate Smad2/3/4 expression in HT-29 colon cancer cells
  • Mohammad Ali Majdoddin,1 Fatemeh Safari,2,*
    1. Tehran Payam noor University
    2. Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran


  • Introduction: One of the main causes of mortality worldwide is colon cancer. It was shown that the current cancer therapy platforms are not effective and thereby, exploring new strategies with high effectiveness remains a major challenge. Due to unique biological characteristics of stem cells, it is considered that stem cells is a potent promising platform in cancer therapy. The aim of this study is evaluation of human amniotic mesenchymal stromal cells (hAMSCs) secretome effects on Smad2/3/4 expressions in HT-29 colon cancer cell.
  • Methods: We are interested to evaluate the effects of hAMSCs secretome on Smad2/3/4 expressions in HT-29 colon cancer cells. To do so, we employed a co-culture system using Transwell 6-well plates. After 72h, hAMSCs-treated HT-29 cells, Smad2/3/4 expressions were analyzed using western blot method.
  • Results: The significant down regulation of Smad2/3/4 expressions in HT-29 cells after 72h treatment with hAMSCs by using western blot was demonstrated.
  • Conclusion: Our results support the idea that hAMSCs may be a unique and effective therapeutic approach to treat colon cancer cells.
  • Keywords: HT-29 colon cancer cells, hAMSCs, Smad2/3/4 expression