Investigating the interaction and expression level of YWHAE mRNA and lncRNA CCAT1 in AML cancer
Investigating the interaction and expression level of YWHAE mRNA and lncRNA CCAT1 in AML cancer
Seyedeh Elina Hosseinipour,1Azizeh Asadzadeh,2,*Bita Behboodian,3
1. . Department of Biology, Faculty of Basic Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran 2. Department of Biology, Faculty of Basic Sciences, Nourdanesh Institute of Higher Education, Meymeh, Isfahan, Iran 3. Department of Animal Science, Kashmar Branch, Islamic Azad University, Kashmar, Iran
Introduction: Introduction: A kind of blood cancer that targets the bone marrow and blood cells is known as acute myeloid leukemia (AML), also referred to as acute myelogenous leukemia. The disease signs and symptoms include weariness, infections, and bleeding issues. In recent years, the vital role of long non-coding RNAs (lncRNAs) in tumor formation and progression has been widely investigated. One of the most important LncRNAs known to be associated with tumor development is CCAT1. Considering the importance of the multifunctional protein YWHAE in signal transduction, cell cycle regulation, and apoptosis, the purpose of this study is to investigate the interaction and expression level of YWHAE mRNA and lncRNA CCAT1 in AML cancer, based on the bioinformatic studies.
Methods: Methods: In order to check the expression change of YWHAE and CCAT1 genes, in acute myeloid leukemia, we used the GEO website (Gene Expression Omnibus ). By mRNA expression datasets in this site, we analyzed the differentially expressed genes (DEGs) related to AML cancer. In the next step to confirm the data, we used Gene Expression Profiling Interactive Analysis (GEPIA) and previous researches. After data validation, Web Server LncRRIsearch was used to investigate the interaction of YWHAE mRNA and lncRNA CCAT1. For further analysis, we obtained the 3D structure of YWHAE protein (from the PDB website https://www.rcsb.org/) and we prepared YWHAE protein for AutoDock and obtained the 3D structure of lncRNA (using 3D RNA/DNA webserver http://biophy.hust.edu.cn/new/). Finally, we showed the interaction between CCAT1 LNCRNA and YWHAE protein by discovery.
Results: Results: Data from the GEO website analysis revealed that CCAT1 was up-regulated gene and YWHAE was downregulated gene in AML cancer. For the YWHAE protein, in the GEPIA database, 173 patient samples and 70 healthy samples were investigated, and based on the produced box plot diagram, we saw that the expression of the YWHAE protein was reduced in this cancer. Our research revealed that the energy binding of YWHAE mRNA and lncRNA CCAT1 is -16.36 Kcal/mol. Finally, analyzing the docking showed YWHAE protein is capable to interact with lncRNA CCAT1 too.
Conclusion: Conclusion: Our results showed that YWHAE mRNA interacts with CCAT1 LncRNA, and this can be the reason for the decrease in YWHAE expression.