Curcumin ameliorates cisplatin-induced pancreas toxicity in diabetic rats
Curcumin ameliorates cisplatin-induced pancreas toxicity in diabetic rats
Nilufar Lotfian,1,*Zeinab Shafiei,2Dian Dayer,3Vahid Bayati,4Esrafil Mansouri,5
1. Cellular and Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran 2. Cellular and Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran 3. Cellular and Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran 4. Cellular and Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran 5. Cellular and Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Introduction: Cisplatin therapy as the most common potent chemotherapeutic process is accompanied by side
effects. Inflammatory mechanisms may play an important role in the pathogenesis of Cisplatin-
induced pancreas toxicity in diabetic rats. Curcumin is an orange-yellow polyphenol present in
curry spice and has anti-inflammatory and antioxidant effects. The current study was planned to
investigate the effect of Curcumin on CP-induced pancreas toxicity in Streptozocin (STZ)-
induced diabetic rats.
Methods: A total of 32 normal male rats were chosen and randomly divided into four groups: Group A or
control received no treatment, Group B received STZ, Group C received STZ + Cisplatin, and
Group D received STZ + Cisplatin + Curcumin. Diabetes was induced by a single intraperitoneal
injection of STZ (60 mg/kg). Rats were given one dose of 7 mg/kg Cisplatin intraperitoneally.
Curcumin treatment involved one dose of 200 mg/kg Curcumin injection intraperitoneally.
Serum and tissue samples were harvested for biochemical, and histopathological investigations.
The concentration of Glucose and the Lipase and Catalase activities were determined using the
photometric method. The Amylase and glutathione peroxidase enzyme activities were evaluated
in a kinetic manner. The levels of IL-1β and IL-6 were measured by ELISA. The contents of
MDA and SOD were calculated using a fluorometry assay.
Results: Cisplatin administration in diabetic rats resulted in significantly elevated serum levels of blood
Glucose, Amylase and Lipase (P<0.05). Coadministration of Cisplatin and Curcumin had an
important reducing effect on Glucose, amylase and lipase compared to the rats treated with
Cisplatin alone (P<0.01). The diabetic rats treated with Cisplatin presented significantly elevated
levels of MDA(P<0.01). Curcumin ameliorated the elevated levels of MDA (P<0.01). Moreover,
Curcumin-therapy resulted in elevated levels of Superoxide dismutase, Catalase, and Glutathione
peroxidase enzyme activities (P<0.05). A noticeable elevation in TNF-α, IL-6, and IL-1β levels
was observed following Curcumin administration in diabetic rats (P<0.001). The results of
histopathological experiments confirmed the anti-inflammatory and antioxidant effects the
Curcumin in diabetic rats.
Conclusion: This study highlights the potential role of Curcumin against Cisplatin-induced toxicity in diabetic
rats, exhibited through favourable alterations in biochemical and histological changes as well as
a reduction in oxidative stress and cytokine levels.