key altered biological pathways in acute myeloid leukemia with and without FLT3 mutation
key altered biological pathways in acute myeloid leukemia with and without FLT3 mutation
Zeinab Ghadimi Nejad Anari,1,*Zahra Rostami,2
1. Faculty of Biological Science and Technology, University of Science and Culture 2. Faculty of Biological Science and Technology, University of Science and Culture
Introduction: FLT3 is one of the significant genes involved in Acute Myeloid Leukemia (AML), its activation results in apoptosis, proliferation, and differentiation of hematopoietic cells. It has two mutation hotspots which leads to constitutive activation and causing AML. The survival rate for FLT3 related AML is nearly double fold lower than wild type AML.
Methods: In this study, the dataset with GEO accession number GSE17855 has been used, the data analysis progressed with R using 50 wild type AML samples and 48 FLT3-related AML. The p-value was set on 0.01, resulted in 5000 genes. We Obtained approximately 200 hub genes using STRING and Cytoscape 3.6.0. once again using STRING and Gephi 0.9.5 the hubs of hub genes and its modules were obtained. After surveying genes on Enrichr, the joint pathways of five databases were obtained and analyzed.
Results: Top five down regulated genes were CTNNB1, UBB, UBE21, BIRC5 and FOXO3, and top five up regulated genes were JUN, EP300, TP53, FOS and MAPK14. These genes were mostly involved in CCKR signaling map, MAPK Family Signaling.
Conclusion: The treatment of patients with AML-FLT3, which is now done by inhibiting FLT3, has some complications; this study focuses on specifying downstream pathways which may help for more effective therapies.