CHAuNP affect the human colorectal adenocarcinoma cell line's clonogenicity and mitochondrial membrane potential (Δψm)
CHAuNP affect the human colorectal adenocarcinoma cell line's clonogenicity and mitochondrial membrane potential (Δψm)
Javad Jodat,1,*Mohammad Ali Jalalifar,2Alireza Ekrami,3Dian Dayer,4
1. Department of Laboratory Sciences, School of Allied Medical Sciences, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran 2. Thalassemia and Hemoglobinopathy Research Center, Research Institute of Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran 3. Cellular and Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran 4. Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Introduction: In the world, CRC is the third most common cancer that kills people of both sexes.
Nanoparticles have been introduced as a promising and potentially effective approach for the treatment of
tumors as a result of the use of nanotechnology in medicine. Gold nanoparticles have special
physicochemical characteristics that allow them to influence critical cancer cell activities.
Methods: We employed the human colorectal adenocarcinoma cell line HT-29 for this
study. covering chitosan Gold nanoparticles were synthesized chemically. The size distribution, surface
plasmon resonance, and imaging were analyzed using DLS, visible-ultraviolet (UV-VIS) spectroscopy,
and atomic force microscopy, respectively. Cell survival and metabolic activity were assessed using the
MTT test, and the IC-50 of gold nanoparticles was determined. Flow cytometry Annexin V-FITC / PI was
used to evaluate apoptosis. The mitochondrial membrane potential (Δψm)was measured using
Rhoudamin123. Reactive oxygen species were measured using the DCFH-DA method (ROS). Colony
formation capacity was evaluated using a clonogenic assay method. The relative expression of Gene
expression was quantified using the real-time qRCR technique.
Results: The chitosan-coated gold nanoparticles were spherical in shape, with an average size of 12.3 nm
and a surface plasmon resonance at 520 nm. 33 μM was the evaluated IC-50 for gold nanoparticles. In
comparison to the control group, the nanoparticles markedly limit colony formation, diminish
mitochondrial membrane potential, and trigger apoptosis in HT-29 cells. Additionally, there was no
significant alteration in the level of reactive oxygen species produced. Genes involved in apoptosis and
anti-apoptosis respectively showed a significant increase and decrease in their relative expression.
Conclusion: The evidence suggests that gold nanoparticles coated with chitosan have a cytotoxic effect
on human colorectal adenocarcinoma, indicating that they can be further investigated as a therapeutic
agent.