Detection of PDK1 gene expression difference and clinicopathology of samples in paraffin block tissues of gastric adenocarcinoma
Detection of PDK1 gene expression difference and clinicopathology of samples in paraffin block tissues of gastric adenocarcinoma
Dr.Faramak Jamshidian,1Dr.Shohre Zare,2,*
1. Department of Biology , East Tehran Branch , Islamic Azad University , Tehran , Iran 2. Department of Genetics and Biology , School of Biological Science , Varamin-Pishva Branch , Islamic Azad University , Varamin , Iran
Introduction: Gastric cancer (GC) is a multifactorial disease, where many factors can influence its development, both environmental and genetic. Current statistics display GC as the fourth leading cause of cancer deaths worldwide, where the rate of median survival is less than 12 months for the advanced stage. Gastric carcinoma as a malignancy of a high aggressiveness with its heterogenous nature, and still constitutes a global health problem. That is why alternative prevention, considered as a proper diet, early diagnosis and follow-up proper treatments, leads to the reduction of recorded incidents. In this research, we will investigate the difference in the expression of PDK1 gene in the tissues of healthy people or tumors with stomach cancer in order to determine the diagnostic value of PDK1.
Methods: In this study, after collecting samples, RNA extraction and cDNA synthesis were performed. Then, we used the Real-time PCR technique to check the gene expression, and the data obtained from it were analyzed through Rest2009 software.
Results: Finally, after analyzing the data obtained from the Real Time PCR reaction, it was found that the PDK1 gene had a significant difference in expression between healthy and tumor tissue, so that its increased expression in tumor tissue was confirmed.
Conclusion: The significant expression difference of PDK1 and the obtained data show that this gene can be introduced as a gastric cancer biomarker. More research should be done to be sure.